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Miltenyi Biotec-tested panel 14 (MBTP 14)
This application protocol describes the flow cytometric analysis of macrophages after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|Viobility™ 405/520 Fixable Dye||-||Viability||-||525/50 (violet)|
|F4/80||REA126||Red pulp macrophages||FITC||525/50 (blue)|
|CD68||REA835||Red pulp macrophages||PE||585/40 (blue)|
|CD3ε||REA606||Cell type exclusion||PE-Vio® 615||655-730 (blue)|
|CD19||REA749||Cell type exclusion||PE-Vio® 615||655-730 (blue)|
|CD335 (NKp46)||REA815||Cell type exclusion||PE-Vio® 615||655-730 (blue)|
|CD64||REA286||Red pulp macrophages||PE-Vio 770||750LP (blue)|
|CD45||REA737||Leukocytes||APC-Vio 770||750LP (red)|
|Mer||REA477||Red pulp macrophages||APC||655-730 (red)|
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