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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Stimulated sample |
Before enrichment | After enrichment |
Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Unstimulated control |
Before enrichment | After enrichment |
Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
* Percentage represents frequency among CD4+ T cells. |
** Percentage represents frequency among enriched cells. |
BALB/c mice were immunized i.p. with keyhole limpet hemocyanin (KLH) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2- secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. |
Stimulated sample | Unstimulated control |
Figure 2BALB/c mice were immunized i.p. with keyhole limpet hemocyanin (KLH) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2- secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. | Figure 2BALB/c mice were immunized i.p. with keyhole limpet hemocyanin (KLH) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2- secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. |
* Percentage represents frequency among CD4+ T cells. |
BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Stimulated sample |
Before enrichment | After enrichment |
Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
Unstimulated control |
Before enrichment | After enrichment |
Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. | Figure 1BALB/c mice were immunized and the spleen cells restimulated with HEL as described for the Mouse IFN-γ Secretion Assay. The responding cells were stained and enriched according to secretion of IL-2 using the Mouse IL-2 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 3125 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, only 720 IL-2-secreting CD4 + T cells were enriched per 10 6 CD4 + T cells. |
* Percentage represents frequency among CD4+ T cells. |
** Percentage represents frequency among enriched cells. |
BALB/c mice were immunized i.p. with keyhole limpet hemocyanin (KLH) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2- secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. |
Stimulated sample | Unstimulated control |
Figure 2BALB/c mice were immunized i.p. with keyhole limpet hemocyanin (KLH) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2- secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. | Figure 2BALB/c mice were immunized i.p. with keyhole limpet hemocyanin (KLH) in incomplete Freund's adjuvant and pertussis toxin. On day 21 after immunization, mouse spleen cells were restimulated in vitro with KLH for 15 hours. The responding cells were stained for coexpression of cytokines using the Mouse IFN-γ Secretion Assay - Detection Kit (PE) and Mouse IL-2 Secretion Assay - Detection Kit (APC). The plots show IFN-γ- and IL-2- secreting cells gated on viable CD4 + T cells on the stimulated sample as well as on the unstimulated control. |
* Percentage represents frequency among CD4+ T cells. |
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