KIR Typing Kits

KIR Typing Kits

The KIR Typing Kit allows the detection of human killer cell immunoglobulin-like receptors (KIRs) on genomic DNA level or the analysis of KIR expression on mRNA level.
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The presence or absence of KIR genes is analyzed by PCR using carefully designed sequence-specific primers (SSPs), enabling the detection of all known 15 human KIR genes plus two pseudo genes. Allel coverage: IPD KIR Sequence database release 2.1, March 2009 (http://www.ebi.ac.uk/ipd/)
Each well of the 96-well PCR plate contains a lyophilized enzyme mix, including Taq DNA Polymerase, as well as positive control primers. Resuspension Buffer is simply mixed with the template and dispensed into the wells of the PCR plate. PCR products are ready for immediate analysis by electrophoresis due to the integrated gel loading buffer.

Background information

KIRs can be found on natural killer (NK) cells which play an important role in the innate immune response. NK cells have been implicated in the suppression of graft-versus-host disease, promotion of bone marrow engraftment, and mediation of a graft-versus-leukemia effect. KIRs exist in various isoforms. The inhibitory KIRs interact with specific HLA class I molecules, predominantly HLA-C, on target cells.
Not all KIR genes are present in every human individual. Further heterogeneity exists at the transcriptional level. Different subsets of NK cells express different KIRs, even within one individual.

Downstream applications

The KIR Typing Kit allows the investigation of the KIR genotype on the DNA level and its expression on mRNA level. The KIR Typing Kit has been used, for example, for KIR profiling after haploidentical stem cell transplantation and for research into the role of KIR genotypes in susceptibility to infectious disease.
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  • Selected references

    1. Basu, D. et al. (2009) Stimulatory and inhibitory killer Ig-like receptor molecules are expressed and functional on lupus T cells. J. Immunol. 183: 3481-3487
    2. Pérez-Martínez, A. et al. (2009) KIR-HLA receptor-ligand mismatch associated with a graft-versus-tumor effect in haploidentical stem cell transplantation for pediatric metastatic solid tumors. Pediatr. Blood Cancer 53: 120-124
    3. André, M. C. et al. (2010)
      Long-term human CD34
      +
      stem cell-engrafted nonobese diabetic/SCID/IL-2R gamma(null) mice show impaired CD8
      +
      T cell maintenance and a functional arrest of immature NK cells.
      J. Immunol. 185: 2710-2720
    4. Siegler, U. et al. (2010) Good manufacturing practice-compliant cell sorting and large-scale expansion of single KIR-positive alloreactive human natural killer cells for multiple infusions to leukemia patients. Cytotherapy 12(6): 750-763
    5. Wauquier, N. et al. (2010) Association of KIR2DS1 and KIR2DS3 with fatal outcome in Ebola virus infection. Immunogenetics 62: 767-771
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  • Special protocols

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Product options: 2
130-092-551

KIR Typing Kit

for 8
tests
SGD 557.00
130-092-584

KIR Typing Kit

for 24
tests
SGD 1,430.00