REAlease® CD94 Complexes, human

REAlease
®
CD94 Complexes
, human

Clone: REAL287 | Dilution: 1:50
Clone REAL287 is an antibody fragment derived from the full CD94 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL287 recognizes CD94, an approximately 45 kDa, single-pass, type II transmembrane protein, which belongs to C-type lectin superfamily. CD94 associates covalently with NKG2 family members and forms disulfide bonded heterodimers. CD94-NKG2 receptors bind HLA-E, the non-classical MHC class Ib molecule. Depending on the associated NKG2 member, CD94-NKG2 heterodimer transmits either activating or inhibitory signal. For, e.g, via immunoreceptor tyrosine based-inhibitory motifs (ITIMs) containing NKG2A, CD94-NKG2A heterodimers inhibit NK cell cytotoxicity. Besides NK cells, expression of CD94-NKG2 heterodimers is also shown on NK-T cells and a small percentage of CD8 cells in mice, where the crosslinking of CD94-NKG2 was shown to reduce the level of spontaneous apoptosis.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

KLRD1, Kp43

Technical specifications

  • Antigen: CD94
  • Clone: REAL287
  • Isotype control: REAL Control
  • Alternative names of antigen: KLRD1, Kp43
  • Distribution of antigen: NK cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, APC
  • Selected references

    1. Brooks, A. G. et al. (1997) NKG2A complexed with CD94 defines a novel inhibitory natural killer cell receptor. J. Exp. Med. 185: 795-800
    2. Braud, V. M. et al. (1998) HLA-E binds to natural killer cell receptors CD94/NKG2A, B and C. Nature 391(6669): 795-799
    3. Gunturi, A. et al. (2003) Preferential survival of CD8 T and NK cells expressing high levels of CD94. J. Immunol. 170: 1737-1745
    4. Wolpert, F. et al. (2015) Interferon-β modulates the innate immune response against glioblastoma initiating cells. PLoS One 10(10): e0139603
    5. Canté-Barrett, K. et al. (2017)
      Loss of CD44
      dim
      expression from early progenitor cells marks T-cell lineage commitment in the human thymus.
      Front Immunol 8: 32
Product options: 6
130-116-331

REAlease CD94-FITC, human

for 30
tests
(1)
SGD 235.00
130-116-231

REAlease CD94-FITC, human

for 100
tests
(1)
SGD 580.00
130-116-327

REAlease CD94-APC, human

for 30
tests
(1)
SGD 290.00
130-116-225

REAlease CD94-APC, human

for 100
tests
(1)
SGD 695.00
130-122-724

REAlease CD94-FITC Kit, human

for 100
tests
(1)
SGD 695.00
130-122-725

REAlease CD94-APC Kit, human

for 100
tests
(1)
SGD 815.00