CD62L antibodies, mouse

CD62L antibodies, mouse

Clone: REA828 | Dilution: 1:50
Clone REA828 recognizes mouse CD62L, also known as L-selectin. CD62L is a member of the selectin family of cell adhesion molecules. CD62L is important for the homing of lymphocytes to peripheral lymph nodes through high endothelial venules. It also contributes to leukocyte emigration into acute inflammatory sites. CD62L is a glycoprotein expressed on the cell surface of most thymocytes, T cells, B cells, monocytes, dendritic cells, neutrophils, and eosinophils. It is highly expressed on naive T cells and down-regulated upon differentiation due to activation-dependent shedding. CD62L is also expressed on a subset of CCR7
high
CD44
high
central memory T cells and on CD4
+
CD25
+
regulatory T cells.
Additional information: Clone REA828 displays negligible binding to Fc receptors.

Alternative names

Sell, L-selectin, LECAM-1, Lnhr, Ly-22, Ly-m22, Lyam-1, Lyam1, LAM-1

Technical specifications

  • Antigen: CD62L
  • Clone: REA828
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: Sell, L-selectin, LECAM-1, Lnhr, Ly-22, Ly-m22, Lyam-1, Lyam1, LAM-1
  • Entrez Gene ID: 20343
  • Molecular mass of antigen [kDa]: 38
  • Distribution of antigen: B cells, bone marrow, eosinophils, granulocytes, Langerhans cells, lymphocytes, monocytes, neutrophils, NK cells, red blood cells, spleen, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC, VioBlue, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA828 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA828
MEL14++
95218-
  • Selected references

    1. Siegelman, M. H. et al. (1990) The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding. Cell 61(4): 611-622
    2. Gallatin, W. M. et al. (2006) A cell-surface molecule involved in organ-specific homing of lymphocytes. J. Immunol. 177(1): 5-9
Product options: 18
130-112-835

CD62L-FITC, mouse

30 µg in 200 µL (1)
SGD 90.00
130-112-646

CD62L-FITC, mouse

150 µg in 1 mL (1)
SGD 265.00
130-112-836

CD62L-PE, mouse

30 µg in 200 µL (1)
SGD 170.00
130-112-647

CD62L-PE, mouse

150 µg in 1 mL (1)
SGD 500.00
130-112-837

CD62L-APC, mouse

30 µg in 200 µL (1)
SGD 170.00
130-112-648

CD62L-APC, mouse

150 µg in 1 mL (1)
SGD 500.00
130-112-841

CD62L-VioBlue, mouse

30 µg in 200 µL (1)
SGD 210.00
130-112-652

CD62L-VioBlue, mouse

150 µg in 1 mL (1)
SGD 605.00
130-112-842

CD62L-PE-Vio615, mouse

30 µg in 200 µL (1)
SGD 225.00
130-112-653

CD62L-PE-Vio615, mouse

150 µg in 1 mL (1)
SGD 645.00
130-112-838

CD62L-PE-Vio770, mouse

30 µg in 200 µL (1)
SGD 225.00
130-112-649

CD62L-PE-Vio770, mouse

150 µg in 1 mL (1)
SGD 645.00
130-112-839

CD62L-APC-Vio770, mouse

30 µg in 200 µL (1)
SGD 225.00
130-112-650

CD62L-APC-Vio770, mouse

150 µg in 1 mL (1)
SGD 645.00
130-112-840

CD62L-PerCP-Vio700, mouse

30 µg in 200 µL (1)
SGD 225.00
130-112-651

CD62L-PerCP-Vio700, mouse

150 µg in 1 mL (1)
SGD 645.00
130-112-834

CD62L-Biotin, mouse

30 µg in 200 µL (1)
SGD 90.00
130-112-645

CD62L-Biotin, mouse

150 µg in 1 mL (1)
SGD 265.00