CD25 antibodies, mouse

CD25 antibodies, mouse

Clone: REA568 | Dilution: 1:50
Clone REA568 recognizes the mouse CD25 antigen, the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4
+
CD25
+
regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. In combination with CD4, CD25 antibody can be used to identify regulatory T cells (Tregs).
Additional information: Clone REA568 displays negligible binding to Fc receptors.

Alternative names

IL2RA, IL2R, Ly-43, p55

Technical specifications

  • Antigen: CD25
  • Clone: REA568
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: IL2RA, IL2R, Ly-43, p55
  • Entrez Gene ID: 16184
  • Molecular mass of antigen [kDa]: 28
  • Distribution of antigen: T cells, B cells, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBright 667, VioBright FITC, PE, APC
  • Selected references

    1. Miller, J. et al. (1985) Nucleotide sequence and expression of a mouse interleukin 2 receptor cDNA. J. Immunol. 134(6): 4212-4217
    2. Read, S. et al. (2000)
      Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25
      +
      CD4
      +
      regulatory cells that control intestinal inflammation.
      J. Exp. Med. 192(2): 295-302
    3. Taniguchi, T. et al. (1993) The IL-2/IL-2 receptor system: a current overview. Cell 73(1): 5-8

REAfinity clone is tested against different known clones, in a blocking experiment, to identify whether they recognize completely overlapping (++), partially overlapping (+), or completely different epitopes (-). In a blocking experiment, cells are incubated with an excess of purified unconjugated REAfinity clone followed by staining with conjugated form of the other known clones. 

Other clonesOverlap in epitope recognition with REA568
3C7+
7D4++
PC61-
Product options: 8
130-118-376

CD25-VioBright 667, mouse

30 µg in 200 µL (1)
SGD 315.00
130-118-222

CD25-VioBright 667, mouse

150 µg in 1 mL (1)
SGD 920.00
130-120-172

CD25-VioBright FITC, mouse

30 µg in 200 µL (1)
SGD 225.00
130-120-088

CD25-VioBright FITC, mouse

150 µg in 1 mL (1)
SGD 645.00
130-120-766

CD25-PE, mouse

30 µg in 200 µL (1)
SGD 170.00
130-120-696

CD25-PE, mouse

150 µg in 1 mL (1)
SGD 500.00
130-120-767

CD25-APC, mouse

30 µg in 200 µL (1)
SGD 170.00
130-120-697

CD25-APC, mouse

150 µg in 1 mL (1)
SGD 500.00