CD25 antibodies, human

CD25 antibodies, human

Clone: REA945 | Dilution: 1:50
Clone REA945 recognizes the human CD25 antigen, a 28 kDa glycoprotein also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4
+
regulatory T cells. CD25 plays an essential role in the regulation of immune tolerance by controlling regulatory T cells activity. The CD25 antigen contains three epitope regions called A, B, and C. Clone REA945 recognizes epitope region B.
Additional information: Clone REA945 displays negligible binding to Fc receptors.

Alternative names

IL2RA, IDDM10, IL2R, TCGFR, p55, Tac, IL-2Rα

Technical specifications

  • Antigen: CD25
  • Clone: REA945
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: IL2RA, IDDM10, IL2R, TCGFR, p55, Tac, IL-2Rα
  • Entrez Gene ID: 3559
  • Molecular mass of antigen [kDa]: 28
  • Cross-reactivity:
    cynomolgus monkey (
    Macaca fascicularis
    )
    ,
    rhesus monkey (
    Macaca mulatta
    )
  • Distribution of antigen: myeloid cells, B cells, granulocytes, lymphocytes, macrophages, monocytes, NK cells, osteoclasts, T cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio615, PE-Vio770, VioBright 515, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA945 with other known clones, a competition assay was performed.
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA945
4E3++
3G10-
REA570-
2A3-
BC96-
M-A251++
  • Selected references

    1. Sakaguchi, S. et al. (1995) Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor α-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases. J. Immunol. 155: 1151-1164
    2. Shevach, E. M. (2001)
      Certified professionals: CD4
      +
      CD25
      +
      suppressor T cells.
      J. Exp. Med. 193(11): F41-F46
    3. Maloy, K. J. et al. (2001) Regulatory T cells in the control of immune pathology. Nat. Immunol. 2(9): 816-822
    4. Lamprecht, B. et al. (2008) Aberrant expression of the Tʜ2 cytokine IL-21 in Hodgkin lymphoma cells regulates STAT3 signaling and attracts Treg cells via regulation of MIP-3alpha. Blood 112(8): 3339-3347
    5. Chaput, N. et al. (2013)
      Phase I clinical trial combining imatinib mesylate and IL-2: HLA-DR
      +
      NK cell levels correlate with disease outcome.
      Oncoimmunology 2(2): e23080
Product options: 11
130-115-628

CD25-PE, human

for 30
tests
(1)
SGD 210.00
130-115-534

CD25-PE, human

for 100
tests
(1)
SGD 510.00
130-115-535

CD25-APC, human

for 100
tests
(1)
SGD 510.00
130-115-631

CD25-PE-Vio615, human

for 30
tests
(1)
SGD 265.00
130-115-537

CD25-PE-Vio615, human

for 100
tests
(1)
SGD 630.00
130-116-308

CD25-PE-Vio770, human

for 30
tests
(1)
SGD 265.00
130-116-205

CD25-PE-Vio770, human

for 100
tests
(1)
SGD 630.00
130-115-630

CD25-VioBright 515, human

for 30
tests
(1)
SGD 330.00
130-115-536

CD25-VioBright 515, human

for 100
tests
(1)
SGD 790.00
130-115-626

CD25-Biotin, human

for 30
tests
(1)
SGD 160.00
130-115-532

CD25-Biotin, human

for 100
tests
(1)
SGD 380.00