Anti-TRA-1-81 Live Cell Stain antibodies, human

Anti-TRA-1-81 Live Cell Stain antibodies, human

Clone: REA246
Anti-TRA-1-81 Live Cell Stain antibodies have been developed for the detection of TRA-1-81
+
cells directly in culture without the need for fixation, e.g., to facilitate colony picking after reprogramming or to evaluate pluripotency of a culture directly in the cell culture dish.
Clone REA246 recognizes a carbohydrate epitope expressed on podocalyxin, which is a transmembrane sialoglycoprotein. Podocalyxin is a member of the CD34-related family of sialomucins and is recognized as a stem cell and malignancy marker. The expression of TRA-1-81 antigen is stage-specific and is lost upon differentiation of human EC and ES cells, making it a marker of undifferentiated pluripotent ES cells.
Additional information: Clone REA246 displays negligible binding to Fc receptors.

Alternative names

PODXL, Gp200, PC, PCLP, PCLP-1, Podocalyxin

Technical specifications

  • Antigen: TRA-1-81
  • Clone: REA246
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: PODXL, Gp200, PC, PCLP, PCLP-1, Podocalyxin
  • Entrez Gene ID: 5420
  • Molecular mass of antigen [kDa]: 56
  • Distribution of antigen: ES and iPS cells
  • Endotoxin level: Low endotoxin levels as determined by LAL assay.
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates:
  • Selected references

    1. Schopperle, W. M. et al. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25(3): 723-730
    2. Abujarour, R. et al. (2013) Optimized surface markers for the prospective isolation of high-quality hiPSCs using flow cytometry selection. Sci. Rep. 3: 1179
Product options: 1
130-106-873

Anti-TRA-1-81-Vio488 Live Cell Stain, human

for
up to
10 mL_medium
SGD 578.00