Anti-TNF-α antibodies, human

Anti-TNF-α antibodies, human

Clone: REA656 | Dilution: 1:50
Clone REA656 recognizes the human tumor necrosis factor alpha (TNF-α) antigen, which is a cytokine involved in inflammatory immune responses. TNF-α has different functions, it plays an important role in the regulation of immune cells and is a potent pyrogen causing fever and apoptotic cell death. Furthermore, the TNF intracellular domain form is able to induce interleukin 12 (IL-12) production in dendritic cells. TNF-α is secreted by activated CD4
+
T cells, monocytes, macrophages, natural killer (NK) cells, mast cells, eosinophils, and neutrophils.
Additional information: Clone REA656 displays negligible binding to Fc receptors.

Alternative names

Tumor necrosis factor, Cachectin, TNF-alpha, TNF-a, DIF-alpha, TNFa, TNFSF2

Technical specifications

  • Antigen: TNF-α
  • Clone: REA656
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: Tumor necrosis factor, Cachectin, TNF-alpha, TNF-a, DIF-alpha, TNFa, TNFSF2
  • Entrez Gene ID: 7124
  • Molecular mass of antigen [kDa]: 26
  • Distribution of antigen: T cells, monocytes, macrophages, NK cells, mast cells, eosinophils, neutrophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC

Specificity

In order to compare the epitope specificity of REAfinity Clone REA656 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA656
cA2++
mab11++
357-167-24-
  • Selected references

    1. Nedospasov, S. A. et al. (1985) Tandem arrangement of genes coding for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) in the human genome. Cold Spring Harb. Symp. Quant. Biol. 51(1): 611-624
    2. Friedmann, E. et al. (2006) SPPL2a and SPPL2b promote intramembrane proteolysis of TNFalpha in activated dendritic cells to trigger IL-12 production. Nat. Cell Biol. 8(8): 843-848
    3. Nedwin, G. E. et al. (1985) Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucleic Acids Res. 13(17): 6361-6373
Product options: 4
130-119-151

Anti-TNF-α-PE, human

for 30
tests
(1)
SGD 235.00
130-118-974

Anti-TNF-α-PE, human

for 100
tests
(1)
SGD 580.00
130-120-149

Anti-TNF-α-APC, human

for 30
tests
(1)
SGD 235.00
130-120-063

Anti-TNF-α-APC, human

for 100
tests
(1)
SGD 580.00