Anti-SSEA-4 antibodies, human

Anti-SSEA-4 antibodies, human

Clone: REA101 | Dilution: 1:11
Clone REA101 recognizes the glycolipid carbohydrate epitope stage specific embryonic antigen 4 (SSEA-4). SSEA-4 is found on undifferentiated human embryonic stem (ES) cells, induced pluripotent (iPS) cells, embryonal carcinoma (EC) cells, as well as embryonic germ (EG) cells and a variety of somatic stem cells, such as dental pulp stem cells, umbilical cord blood–derived very small embryonic like stem cells (VSELs), and mesenchymal stromal cells.
Additional information: Clone REA101 displays negligible binding to Fc receptors.

Technical specifications

  • Antigen: SSEA-4
  • Clone: REA101
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Cross-reactivity: mouse, rabbit, canine, chicken
  • Distribution of antigen: ES and iPS cells, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, VioBlue, VioGreen, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin

Specificity

In order to compare the epitope specificity of REAfinity Clone REA101 with other known clones, a competition assay was performed. 
 Cells were incubated with an excess of purified unconjugated REAfinity Antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.
 

Other clonesOverlap in epitope recognition with REA101
MC813++
  • Selected references

    1. Aloia, A. et al. (2015) The sialyl-glycolipid stage-specific embryonic antigen 4 marks a subpopulation of chemotherapy-resistant breast cancer cells with mesenchymal features. Breast Cancer Res. 17: 146
    2. Gang, E. J. et al. (2007) SSEA-4 identifies mesenchymal stem cells from bone marrow. Blood 109(4): 1743-1751
    3. Truong, T. T. et al. (2011) SSEA4 is a potential negative marker for the enrichment of human corneal epithelial stem/progenitor cells. Invest. Ophthalmol. Vis. Sci. 52(9): 6315-6320
    4. Kannagi, R. et al. (1983) Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside EMBO J. 2(12): 2355-2361
  • Customer reports and application notes

Product options: 13
130-098-371

Anti-SSEA-4-FITC, human

for 100
tests
(1)
SGD 485.00
130-100-635

Anti-SSEA-4-PE, human

for 30
tests
(1)
SGD 235.00
130-098-369

Anti-SSEA-4-PE, human

for 100
tests
(1)
SGD 580.00
130-098-347

Anti-SSEA-4-APC, human

for 100
tests
(1)
SGD 580.00
130-098-366

Anti-SSEA-4-VioBlue, human

for 100
tests
(1)
SGD 605.00
130-098-341

Anti-SSEA-4-VioGreen, human

for 100
tests
(1)
SGD 605.00
130-105-081

Anti-SSEA-4-PE-Vio770, human

for 30
tests
(1)
SGD 265.00
130-105-051

Anti-SSEA-4-PE-Vio770, human

for 100
tests
(1)
SGD 630.00
130-105-082

Anti-SSEA-4-APC-Vio770, human

for 30
tests
(1)
SGD 265.00
130-105-052

Anti-SSEA-4-APC-Vio770, human

for 100
tests
(1)
SGD 630.00
130-105-083

Anti-SSEA-4-PerCP-Vio700, human

for 30
tests
(1)
SGD 265.00
130-105-053

Anti-SSEA-4-PerCP-Vio700, human

for 100
tests
(1)
SGD 630.00
130-098-339

Anti-SSEA-4-Biotin, human

for 100
tests
(1)
SGD 485.00