Anti-PLC-γ2 antibodies, human

Anti-PLC-γ2 antibodies, human

Clone: REA488 | Dilution: 1:11
Clone REA488 recognizes the human phosphoinositide phospholipase C-γ-2 (PLC-γ2) antigen regardless of phosphorylation status. PLC-γ isoforms are mainly regulated through receptors with intrinsic tyrosine kinase activity (e.g. growth factor receptors) or receptors (such as B and T cell–antigen receptors) that are linked to the activation of non-receptor tyrosine kinases through a complex signaling network. The two isoforms of PLC-γ have distinct tissue distributions. Whereas PLC-γ1 is expressed ubiquitously, the pattern of expression of PLC-γ2 is characterized by high levels in cells of hematopoietic origin. PLC-γ2 is a transmembrane signaling enzyme that catalyzes the conversion of phosphatidyl inositol biphosphate to inositol triphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. It has been implicated in collagen-induced signal transduction in platelets and antigen-dependent signaling in B lymphocytes. PLC-γ2 is regulated through activation of tyrosine kinase-linked receptors. Homozygous disruption of PLC-γ2 results in functional and signaling disorders in a subset of cell types including B cells, platelets, and mast cells.
Additional information: Clone REA488 displays negligible binding to Fc receptors.

Alternative names

PLCG2, APLAID, FCAS3, PLC-IV, PLC-gamma-2

Technical specifications

  • Antigen: PLC-γ2
  • Clone: REA488
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: PLCG2, APLAID, FCAS3, PLC-IV, PLC-gamma-2
  • Entrez Gene ID: 5336
  • Molecular mass of antigen [kDa]: 148
  • Cross-reactivity: mouse
  • Distribution of antigen: B cells, hematopoietic stem cells, leukocytes, monocytes, macrophages, NK cells, platelets, lymphocytes, T cells, T helper cells, thymocytes, NKT cells, basophils, eosinophils, mast cells, neutrophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, APC
  • Selected references

    1. Ohta, S. et al. (1988) Complete cDNA encoding a putative phospholipase C from transformed human lymphocytes. FEBS Lett. 242(1): 31-35
    2. Hikida, M. et al. (2009) PLC-gamma2 is essential for formation and maintenance of memory B cells. J. Exp. Med. 206(3): 681-689
    3. Feng, L. et al. (2012) The effect of PLC-γ2 inhibitors on the growth of human tumour cells. Eur J Med Chem 54: 463-469
Product options: 6
130-107-644

Anti-PLC-γ2-FITC, human

for 30
tests
(1)
SGD 195.00
130-107-600

Anti-PLC-γ2-FITC, human

for 100
tests
(1)
SGD 485.00
130-107-645

Anti-PLC-γ2-PE, human

for 30
tests
(1)
SGD 235.00
130-107-601

Anti-PLC-γ2-PE, human

for 100
tests
(1)
SGD 580.00
130-107-646

Anti-PLC-γ2-APC, human

for 30
tests
(1)
SGD 235.00
130-107-602

Anti-PLC-γ2-APC, human

for 100
tests
(1)
SGD 580.00