Anti-IL-12 (p40/p70) antibodies, mouse

Anti-IL-12 (p40/p70) antibodies, mouse

Clone: REA285 | Dilution: 1:10
Clone REA285 recognizes both, the free and complexed forms (homodimer p80 and heterodimer p70) of the p40 subunit of interleukin-12 (IL-12) as well as of interleukin-23 (IL-23). IL-12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. It acts as a growth factor for activated T and natural killer cells, and has a broad array of biological activities. IL-12 has been found to be important for sustaining a sufficient number of memory and effector Th1 cells to mediate long-term protection to intracellular pathogens. Overexpression of IL-12 was observed in the central nervous system of patients with multiple sclerosis, suggesting a role of IL-12 in the pathogenesis of the disease. IL-23, a heterodimeric cytokine which functions in innate and adaptive immunity, may constitute with IL-17 an acute response to infection in peripheral tissues. It stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. The REA285 antibody does not neutralize the bioactivity of natural or recombinant IL-12.
Additional information: Clone REA285 displays negligible binding to Fc receptors.

Alternative names

IL12A, IL-12p35, IL-12A, Ll12a, P35, Il-12b, Il-12p40, Il12p40, P40

Technical specifications

  • Antigen: IL-12 (p40/p70)
  • Clone: REA285
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: IL12A, IL-12p35, IL-12A, Ll12a, P35, Il-12b, Il-12p40, Il12p40, P40
  • Entrez Gene ID: 16160
  • Molecular mass of antigen [kDa]: 36
  • Distribution of antigen: macrophages, dendritic cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected references

    1. Oppmann, B. et al. (2000) Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 13(5): 715-725
    2. Trinchieri, G. et al. (2003) Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nat. Rev. Immunol. 3(2): 133-146
Product options: 4
130-103-891

Anti-IL-12 (p40/p70)-PE, mouse

9 µg in 300 µL (1)
SGD 60.00
130-103-821

Anti-IL-12 (p40/p70)-PE, mouse

30 µg in 1 mL (1)
SGD 170.00
130-103-892

Anti-IL-12 (p40/p70)-APC, mouse

9 µg in 300 µL (1)
SGD 86.00
130-103-822

Anti-IL-12 (p40/p70)-APC, mouse

30 µg in 1 mL (1)
SGD 170.00