Human FGF-2 IS

Human FGF-2 IS

FGF-2 IS stands for fibroblast growth factor 2 "Improved Sequence", also termed fibroblast growth factor basic (FGF-b) or basic FGF. Human FGF-2 IS is a variant of Human FGF-2 with a proprietary amino acid substitution. FGF-2 IS is protected by patent EP2930181. Human FGF-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human FGF-2 can be used for a variety of applications, including:
  • Stimulation of proliferation and differentiation of several cell types, such as mesenchymal stromal cells, neural cells, and endothelial cells.
  • Long-term maintenance and propagation of undifferentiated embryonic and induced pluripotent stem cells.
  • Differentiation of neural cells starting from embryonic and induced pluripotent stem cell cultures.

Alternative names

basic FGF

Background information

Human FGF-2 belongs to the family of heparin-binding growth factors. It functions as a wide-spectrum mitogenic, angiogenic, and neurotrophic factor and stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells. FGF-2 has been implicated in a multitude of physiological and pathological processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF-2 IS is an engineered FGF-2 variant with increased thermostability and higher resistance to proteases, and retains the same biological properties as naturally occurring FGF-2.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of 3T3 cells (NIBSC 90/712)
  • premium grade: ≥ 2×
    10
    6
    IU/mg
  • research grade: ≥ 1×
    10
    6
    IU/mg
  • Selected references

    1. Robinson, C. J. and Gaines-Das, R. (1994)
      The international standard for basic fibroblast growth factor (FGF-2); comparison of candidate preparations by
      in vitro
      bioassays and immunoassays.
      Growth Factors 11: 9-16
Product options: 6
130-104-925

Human FGF-2 IS, research grade

E. coli
10 µg
SGD 181.00
130-104-921

Human FGF-2 IS, research grade

E. coli
50 µg
SGD 472.00
130-104-918

Human FGF-2 IS, premium grade

E. coli
10 µg
SGD 255.00
130-104-924

Human FGF-2 IS, premium grade

E. coli
50 µg
SGD 663.00
130-104-922

Human FGF-2 IS, premium grade

E. coli
200 µg
SGD 1,315.00
130-104-923

Human FGF-2 IS, premium grade

E. coli
1000 µg
SGD 3,176.00