BMP-2 stands for bone morphogenetic protein 2. Human BMP-2 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human BMP-2

Figures

Figure 1

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Human BMP-2 activity assay.
The biological activity of Human BMP-2, premium grade, was determined by alkaline phosphatase assay using mouse C2C12 fibroblasts.

Figure 1

Human BMP-2 activity assay.
The biological activity of Human BMP-2, premium grade, was determined by alkaline phosphatase assay using mouse C2C12 fibroblasts.

Figure 2

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SDS-PAGE of Human BMP-2, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human BMP-2, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

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Mass spectrometry analysis (ESI-MS) of Human BMP-2, premium grade. The peak corresponds to the calculated molecular mass of 26058 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Human BMP-2, premium grade. The peak corresponds to the calculated molecular mass of 26058 Da.

Specifications for Human BMP-2

Overview

BMP-2 stands for bone morphogenetic protein 2. Human BMP-2 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human BMP-2 can be used for a variety of applications, including:
  • Study of chondrogenesis and osteogenesis.
  • Differentiation of mesenchymal stem cells into several cell type including osteoblast and cardiac cells.
  • Investigation of TGF-β signaling pathway.

Detailed product information

Background information

Bone morphogenetic protein 2 (BMP-2) is a member of the bone morphogenetic protein family, which is part of the TGF-β superfamily. Together with BMP-7, BMP-2 is a potent stimulator of osteoblast differentiation as well as of chondrocyte proliferation of mesenchymal cells during bone and cartilage formation, and can also induce cardiac and epithelial morphogenesis. BMP-2 is secreted by several cell tissues such as spleen, lung, liver, brain, small intestine, prostate, and ovary, as immature long form, and after proteolytic cleavage it matures into an active disulfide-linked homodimer. BMP-2 signals through heterodimeric receptors, such as BMPR1A, and activates TGF-β signaling cascade and the hedgehog pathway. Besides the morphogenic role, BMP-2 is also crucial during embryonic dorsal-ventral generation and organogenesis. Recombinant BMP-2 is under clinical investigation for treatment of bone degeneration diseases, spinal injuries, and orthodontic pathologies. Mature human mouse and rat BMP-2 share full amino acid sequence identity. Recombinant Human BMP-2 corresponds to the mature form of BMP-2.

Biological activity

  • Alkaline phosphatase assay using mouse C2C12 fibroblasts (NIBSC 93/574)
  • research grade: ≥ 5×
    10
    4
    U/mg
  • premium grade: ≥ 1×
    10
    5
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human BMP-2

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human BMP-2

Publications

  1. Pi, W. et al. (2012) BMP-2 up-regulates PTEN expression and induces apoptosis of pulmonary artery smooth muscle cells under hypoxia PLoS One 7(5): e35283
  2. Hisanaga, S. et al. (2018) PERK-mediated translational control is required for collagen secretion in chondrocytes Sci Rep 8(1): 773
  3. Cheng, H. et al. (2003) Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J Bone Joint Surg Am 85(A8): 1544-1552
  4. Fujimori, K. et al. (2019) Synthesis of Novel Farnesoid X Receptor Agonists and Validation of Their Efficacy in Activating Differentiation of Mouse Bone Marrow-Derived Mesenchymal Stem Cells into Osteoblasts. Molecules 24(22): 4155
  5. Neybecker, P. et al. (2018) In vitro and in vivo potentialities for cartilage repair from human advanced knee osteoarthritis synovial fluid-derived mesenchymal stem cells Stem Cell Res Ther. 9(1): 329
  6. Kikuchi, T. et al. (2020) Thickness-wise growth technique for human articular chondrocytes to fabricate three-dimensional cartilage grafts Regen Ther 14: 119-127

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