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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. |
Stimulated sample |
Stimulated sample | After enrichment |
Figure 1PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. | Figure 1PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. |
Unstimulated control |
Unstimulated control | After enrichment |
Figure 1PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. | Figure 1PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. |
PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. |
Stimulated sample |
Stimulated sample | After enrichment |
Figure 1PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. | Figure 1PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. |
Unstimulated control |
Unstimulated control | After enrichment |
Figure 1PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. | Figure 1PBMCs were stimulated with PMA and Ionomycin or left untreated. Cells were stained and isolated according to secretion of GM-CSF using the GM-CSF Secretion Assay - Cell Enrichment and Detection Kit (PE). The cells were counterstained with CD4-APC. B cells, monocytes and dead cells were excluded according to CD20-PerCP-Vio700, CD14-PerCP-Vio700 and PI-staining respectively. |
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