IL-4 Secretion Assay – Detection Kit (PE), human

IL-4 Secretion Assay – Detection Kit (PE), human

The IL-4 Secretion Assay was developed for the sensitive detection of human IL-4-secreting cells.

Background information

Interleukin-4 (IL-4) is predominantly secreted by activated CD4
memory and effector T
2 cells, basophils, and mast cells. It induces and supports humoral immune responses for the neutralization of extracellular pathogens. The T
2 types of immune mechanisms can also be involved in immunologic disorders, such as IgE-mediated allergy. The IL-4 Secretion Assay (PE) can also be used for two-color cytokine analysis in combination with the IFN-γ Secretion Assay - Detection Kit (FITC) or the IFN-γ, IL-2, or IL-10 Secretion Assay - Detection Kits (APC).

Downstream applications

IL-4 Secretion Assays were used to isolate and analyze Tetanus Toxoid-specific
, allergen-specific
, and autoreactive
T cells from PBMCs, as well as tumor-specific cells from T cell lines
The IL-4 Secretion Assay was also used for isolation of IL-4-secreting human NK cells
, and for detection of IL-4-secreting NKT cells
IL-4-secreting CD8
T cells were isolated in combination with CD8 MultiSort MicroBeads.
  • Selected references

    1. Deepe, G. S. et al. (2009) The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity. J. Immunol. 183(3): 1964-1974
    2. Brosterhus, H. et al. (1999) Enrichment and detection of live antigen-specific CD4(+) and CD8(+) T cells based on cytokine secretion. Eur. J. Immunol. 29: 4053-4059
    3. Akdis et al. (2000) MACS&more 4: 9-10
    4. Oelke, M. et al. (2000)
      Functional characterization of CD8
      antigen-specific cytotoxic T lymphocytes after enrichment based on cytokine secretion: comparison with the MHC-tetramer technology.
      Scand. J. Immunol. 52: 544-549
    5. Deniz, G. et al. (2002) Human NK1 and NK2 subsets determined by purification of IFN-gamma-secreting and IFN-gamma-nonsecreting NK cells. Eur. J. Immunol. 32: 879-884
    6. Akdis, M. et al. (2004) Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. J. Exp. Med. 199: 1567-1575
    7. Araki, M. et al. (2002)
      Tʜ2 bias of CD4
      NKT cells derived from multiple sclerosis in remission.
      Int. Immunol. 15: 279-288
    8. Veldman, C. et al. (2003) Dichotomy of autoreactive Th1 and Th2 cell responses to desmoglein 3 in patients with pemphigus vulgaris (PV) and healthy carriers of PV-associated HLA class II alleles. J. Immunol. 170: 635-642
    9. Schwaiger, S. et al. (2003)
      IL-4-producing CD8
      T cells with a CD62L
      phenotype accumulate in a subgroup of older adults and are associated with the maintenance of intact humoral immunity in old age.
      J. Immunol. 170: 613-619
  • Special protocols

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