Sorting of bacteria without any risk
Within the closed MACSQuant Tyto Cartridge, the formation of biohazardous aerosols or droplets is completely eliminated, making it a completely safe system. This is particularly important with regards to operator safety when working with high-risk material, such as bacteria. In addition, the closed cartridge design excludes problems like sample-to-sample carry-over or the risk of persistent instrument contamination. Due to the single-use disposable cartridge, there is no need for difficult sterilization procedures between subsequent sorts
This experiment provides a proof of principal for safe sorting of very small (E. coli diameter ≈ 1µm, length ≈ 2µm), high-risk material using the MACSQuant Tyto Sorter. GFP-expressing bacteria (E. coli) were sorted from a mixture with wildtype bacteria, resulting in a sort purity of 97%.