CD62P antibodies, mouse

CD62P antibodies, mouse

Clone: REA344 | Dilution: 1:50
Clone REA344 recognizes the mouse CD62P antigen, a 140 kDa single-pass type I membrane protein also known as P-selectin or platelet activation dependent granule-external membrane protein (PADGEM). CD62P is expressed by vascular endothelial cells and platelets. The protein is stored preformed in the Weibel-Palade bodies of endothelial cells and in the α-granules of platelets. Upon stimulation of these cells with a variety of agonists such as thrombin or histamine, P-selectin is rapidly translocated to the cell surface, where it mediates leukocyte-platelet and leukocyte-vascular endothelial cell adhesion. The interaction of P-selectin with mature leukocytes is in part responsible for mediating leukocyte rolling on vascular cells, an early event in leukocyte recruitment at sites of inflammation. In addition to its role as a receptor for mature neutrophils and monocytes, P-selectin also functions as a cell adhesion molecule for leukocyte precursor cells in the bone marrow, including both lineage-restricted clonogenic progenitors and hierarchically more primitive progenitor cells.
Additional information: Clone REA344 displays negligible binding to Fc receptors.

Alternative names

SELP, GMP-140, GRMP, LECAM3, PADGEM, P-selectin

Technical specifications

  • Antigen: CD62P
  • Clone: REA344
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: SELP, GMP-140, GRMP, LECAM3, PADGEM, P-selectin
  • Entrez Gene ID: 20344
  • Molecular mass of antigen [kDa]: 79
  • Distribution of antigen: endothelial cells, platelets
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected references

    1. Sanders, W. E. et al. (1992)
      Molecular cloning and analysis of
      in vivo
      expression of murine P-selectin.
      Blood 80(3): 795-800
    2. Xia, L. et al. (2003) N-terminal residues in murine P-selectin glycoprotein ligand-1 required for binding to murine P-selectin. Blood 101(2): 552-559
    3. Weller, A. et al. (1992) Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha. J. Biol. Chem. 267(21): 15176-15183
    4. Ando, Y. et al. (2016) Platelet supernatant suppresses LPS-induced nitric oxide production from macrophages accompanied by inhibition of NF-κB signaling and increased arginase-1 expression. PLoS One 11: :e0162208
Product options: 1

CD62P-PE, mouse

30 µg in 200 µL (1)
SEK 820,00 

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