CD62E antibodies, mouse

CD62E antibodies, mouse

Clone: REA369 | Dilution: 1:50
Clone REA369 recognizes the mouse CD62E antigen, a single-pass type I membrane glycoprotein also known as E-selectin. CD62E is expressed on endothelial cells and plays a critical role in the recruitment of the appropriate blood cell into specific local sites. It mediates the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with P-selectin glycoprotein ligand 1, which promotes slow rolling and adhesion. CD62E is transcriptionally induced in response to inflammatory cytokines, such as IL-1β and TNFα. It has an important role during hematopoietic stem/progenitor cell trafficking to the bone marrow, and for the maintenance of their quiescent phenotype
in vivo
. Because selectins are also constitutively expressed in the endothelium of certain tissues such as bone marrow even in the absence of inflammatory stimulation, there has been a growing appreciation that these receptors may be important for tissue homeostasis. Elevated cellular levels of CD62E have been documented in various diseases associated with a pro-inflammatory condition, including diabetes, atherosclerosis, rheumatoid arthritis, and cancer. Conversely, therapeutic strategies, which reduce vascular injury, result in significant down-regulation of CD62E gene expression.
Additional information: Clone REA369 displays negligible binding to Fc receptors.

Alternative names

SELE, E-selectin, ELAM-1, Elam, LECAM2

Technical specifications

  • Antigen: CD62E
  • Clone: REA369
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: SELE, E-selectin, ELAM-1, Elam, LECAM2
  • Entrez Gene ID: 20339
  • Molecular mass of antigen [kDa]: 65
  • Distribution of antigen: endothelial cells, bone marrow, other
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected references

    1. Katayama, Y. et al. (2013) Selective E-selectin ligands. Blood 122(24): 3858-3860
    2. Weller, A. et al. (1992) Cloning of the mouse endothelial selectins. Expression of both E- and P-selectin is inducible by tumor necrosis factor alpha. J. Biol. Chem. 267(21): 15176-15183
    3. Laviola, L. et al. (2013) TNFα signals via p66(Shc) to induce E-selectin, promote leukocyte transmigration and enhance permeability in human endothelial cells. PLoS One 8(12): e81930
Product options: 1

CD62E-PE, mouse

30 µg in 200 µL (1)
SEK 820,00 

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