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Human peripheral blood mononuclear cells (PBMCs) were stained with CD307a (FcRL1) antibodies or with the corresponding REA Control (S) antibodies (left image) as well as with CD19 antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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