Anti-IL-5 antibodies, mouse

Anti-IL-5 antibodies, mouse

Clone: TRFK-5 | Dilution: 1:10
Interleukin 5 (IL-5) is pre-dominantly secreted by CD4
T cells. It is involved in a range of allergic reactions and mediates immune reactions against parasites. IL-5 also acts on other cell types such as B cells.
The Anti-IL-5 antibody has been designed for intracellular staining of IL-5–producing cells. Cells can be stimulated for IL-5-production, for example, by polyclonal stimulation with mitogens. For induction of IL-5 production by antigen-specific T cells, cells are restimulated with respective antigen. IL-5 can be accumulated in the cells by addition of secretion inhibitors like brefeldin A. After fixation and permeabilization of the cell sample, IL-5–producing cells can be stained intracellularly with Anti-IL-5 antibodies. Staining of surface markers allows simultaneous flow cytometric analysis of subsets and activation status of the IL-5–producing cells. Magnetically enriched cells can be stained intracellularly for IL-5 production directly on the MACS
Column. This procedure ensures higher sensitivity of detection and minimizes loss of cells during washing procedures. The protocol is useful for cytokine analysis of rare cells, for example, CD4
T cells in HIV patients, or other cell sources than peripheral blood mononuclear cells (PBMCs), e.g., bronchoalveolar lavages or synovial fluids.

Technical specifications

  • Antigen: IL-5
  • Clone: TRFK-5
  • Isotype: rat IgG1κ
  • Isotype control: Rat IgG1 – isotype control antibodies
  • Entrez Gene ID: 16191
  • Molecular mass of antigen [kDa]: 13
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, PE
Product options: 2
30 µg in 1 mL (1)
SEK 820,00 
30 µg in 1 mL (1)
SEK 820,00 

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