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Splenocytes from C57BL/6 mice were either left unstimulated (left image) or stimulated with 0.5 µg/mL ionomycin, 0.5 µg/mL phorbol 12,13-dibutyrate, and 1 µg/mL brefeldin A for 5 hours. Cells were then fixed, permeabilized, and stained with Anti-IL-2 antibodies as well as with CD154 antibodies. CD4+
cells were pre-gated for the analysis. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals.
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