StraightFrom® Buffy Coat CD3 MicroBead Kit, human

Buffy Coat CD3 MicroBead Kit
, human

The StraightFrom
Buffy Coat CD3 MicroBead Kit has been developed for the rapid positive selection of CD3
cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS
Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat.

Background information

The CD3 antigen is associated with the T cell receptor (TCR) heterodimer. CD3 is expressed on all T cells, constituting 15–20% of human peripheral blood leukocytes.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom
Buffy Coat CD3 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS
Separator. After removing the column from the magnetic field, the magnetically retained CD3
cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD3
cells are well suited for further flow cytometric, functional, or molecular analysis.

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Product options: 1
for 1
buffy coat
SEK 5 365,00 

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