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Example of a separation using the REAlease® CD8 MicroBead Kit
T cells were isolated from human PBMCs using the REAlease CD8 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8-PE and analyzed by flow cytometry using the MACSQuant®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD8+
cells"), whereas the label-free CD8+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD8).
A) Cell purity
B) Label-free cells: REAlease Biotin Complex release
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