Diamond CD133 Isolation Kit, human

Diamond CD133 Isolation Kit, human

The Diamond CD133 Isolation Kit was specifically developed for the isolation of highly pure Lin
stem cells from peripheral blood, cord blood, bone marrow, and leukapheresis product by sequential sorting.

Detailed separation procedure

The Diamond CD133 MicroBead Kit includes a lineage depletion followed by a CD133 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD133
cells are isolated from the lineage-negative stem and progenitor cells using CD133 MicroBeads. The resulting population obtained are Lin
stem cells.
Diamond CD133 MicroBeads recognize the epitope CD133/1. For control staining of separated cells, the application of CD133/2 (293C3) antibody is recommended.

Downstream applications

stem cells can be used for many applications, including:
  • gene or miRNA expression profiling
  • characterization of undifferentiated stem cells and their developmental potential
  • stimulation of proliferation and maturation by cytokines
  • HSC-CFU assays
  • adoptive transfer and hematopoietic reconstitution studies


For the first magnetic separation (depletion): LD or autoMACS
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
  • Selected references

    1. Estrada-González, P.K. et al. (2014) Decreased frequency, but normal functional integrity of mesenchymal stromal cells derived from untreated and Imatinib-treated chronic myeloid leukemia patients. Leuk. Res. 5: 594-600
Product options: 1
for 2×
total cells
SEK 7 320,00 

Related products

3 products available