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Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were enriched by using the Mouse IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.
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