PepTivator JCV ST, human

PepTivator JCV ST, human

JCV ST – research grade is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human JC virus (JCV) ST protein (UniProt ID: P03083-1).
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Background information

JCV is a widespread human polyomavirus. Primary infection results in asymptomatic, latent persistence of the virus. Immunodeficiency or immunosuppression allows virus reactivation. The small T antigen (ST) is a protein expressed early in the lytic cycle. It plays an important role in virus replication.

Downstream applications

PepTivator JCV ST has been specifically developed for efficient
in vitro
stimulation of JCV-specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
and CD8
T cells in various applications, including:
  • Detection and analysis of JCV ST–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable JCV ST–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of JCV ST–specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.
Product options: 1
stimulation of
SEK 1 300,00 

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