T cells | Applications | Miltenyi Biotec

Our T cell workflows cover all steps from sample preparation and cell isolation to flow cytometry and cell culture.

Choose one of the following research fields to discover detailed scientific data and application protocols for your T cell workflow.

Activation and expansion of T cells

Regulatory T cells for immunotherapy

Polarization of CD4+ T Helper cells

Antigen-specific T cells

SARS-CoV-2-specific T cells

Virus-specific T cells for immunotherapy

Tumor infiltrating leukocytes

CAR T cell manufacturing

T cell engineering by electroporation and transduction

T cell depletion in graft engineering

Application highlight

Engineered T cells

The CliniMACS Prodigy Platform enables the generation of gene-modified T cells in a standardized and fully automated way. It is a breakthrough in cell manufacturing, a leap to the next generation.

Learn more on the TCT process

Step-by-step protocols for T cell research

Get acquainted with our protocols and antibody panels covering all workflow steps in your T cell research.

Matching:

MBTP 31: Immunophenotyping of human T cells via transcription factor profiling using flow cytometry

Keywords
Flow cytometry and cell analysis
Immunology
human
T cells
Application protocol
Antibody panel

MBTP 26: Immunophenotyping of regulatory T cells from human PBMCs using flow cytometry

Keywords
Flow cytometry and cell analysis
Immunology
human
regulatory T cells
T cells
Application protocol
Antibody panel

Multicolor flow cytometric analysis of regulatory T cells from mouse spleen

Keywords
Sample preparation
Flow cytometry and cell analysis
Immunology
mouse
spleen
T cells
regulatory T cells
Application protocol

TH1, TH2 and TH17 polarization of naive CD4+ mouse T cells

Keywords
Sample preparation
Cell separation
Cell culture
Flow cytometry and cell analysis
Immunology
mouse
spleen
T cells
naive T cells
Th1 cells
Th2 cells
Th17 cells
Application protocol

MBTP 44: Immunophenotyping of CAR T cell effector cytokine production using flow cytometry

Keywords
Flow cytometry and cell analysis
Immunology
T cells
CAR T cells
cytokine-producing cells
Antibody panel
Application protocol

Human CD4+CD25+ regulatory T cell isolation, in vitro suppression assay and analysis

Keywords
Cell separation
Cell culture
Flow cytometry and cell analysis
Immunology
human
PBMCs
T cells
regulatory T cells
Application protocol

Human CD4+CD25+ regulatory T cell isolation, in vitro expansion and analysis

Keywords
Cell separation
Cell culture
Flow cytometry and cell analysis
Immunology
human
PBMCs
T cells
regulatory T cells
Application protocol

In vitro expansion of human Pan T cells

Keywords
Cell separation
Flow cytometry and cell analysis
Cell culture
Immunology
human
PBMCs
T cells
Application protocol

MBTP 4: Immunophenotyping of tumor-specific CD4+ and CD8+ T cells from a human ovarian tumor using flow cytometry

Keywords
Flow cytometry and cell analysis
Immunology
human
tumor
T cells
lymphocytes
CD4+ T cells
CD8+ T cells
Antibody panel
Application protocol

Engineering of CAR NK cells for research use

Keywords
Cell separation
Flow cytometry and cell analysis
Cell culture
human
T cells
CAR NK cells
PBMCs
Application protocol

MBTP 34: Immunophenotyping of CAR T cell persistence and differentiation using flow cytometry

Keywords
Flow cytometry and cell analysis
Immunology
T cells
CAR T cells
Antibody panel
Application protocol

Multicolor flow cytometric analysis of T cell subsets from mouse spleen

Keywords
Sample preparation
Flow cytometry and cell analysis
Immunology
mouse
spleen
T cells
Application protocol

MBTP 11: Immunophenotyping of T cells from mouse spleen using flow cytometry

Keywords
Flow cytometry and cell analysis
Immunology
mouse
spleen
T cells
Antibody panel
Application protocol

MBTP 33: Immunophenotyping of activated T cells within antigen-stimulated human PBMCs using flow cytometry

Keywords
Flow cytometry and cell analysis
Immunology
T cells
human
Antibody panel
Application protocol

MBTP 43: Immunophenotyping of CAR T cell transduction efficiency and viability using flow cytometry

Keywords
Flow cytometry and cell analysis
Immunology
T cells
CAR T cells
Antibody panel
Application protocol

MBTP 28: Immunophenotyping of virus-specific T cells from human blood using flow cytometry

Keywords
Flow cytometry and cell analysis
Immunology
human
T cells
blood
Antibody panel
Application protocol

Engineering of CAR T cells for research use

Keywords
Cell separation
Flow cytometry and cell analysis
Cell culture
human
PBMCs
T cells
CAR T cells
Application protocol

Human CD4+CD25+CD127dim/- regulatory T cell isolation, in vitro expansion and analysis

Keywords
Cell separation
Cell culture
Flow cytometry and cell analysis
Immunology
human
PBMCs
T cells
regulatory T cells
Application protocol

In vitro expansion of mouse CD4+ T cells

Keywords
Sample preparation
Cell separation
Cell culture
Flow cytometry and cell analysis
Immunology
mouse
spleen
T cells
CD4+ T cells
Application protocol

Rapid antigen-specific T cell enrichment (Rapid ARTE)

Keywords
Cell separation
Cell culture
Flow cytometry and cell analysis
Immunology
human
PBMCs
T cells
CD4+ T cells
Application protocol

Applications for T cell research and immunotherapy