Our T cell workflows cover all steps from sample preparation and cell isolation to flow cytometry and cell culture.
Choose one of the following research fields to discover detailed scientific data and application protocols for your T cell workflow.
Activation and expansion of T cells
Regulatory T cells
Regulatory T cells for immunotherapy
Polarization of CD4+ T Helper cells
Antigen-specific T cells
SARS-CoV-2-specific T cells
Virus-specific T cells for immunotherapy
Tumor infiltrating leukocytes
Engineered T cells for research
TCR / CAR T cell manufacturing
T cell engineering by electroporation and transduction
T cell depletion in graft engineering
Step-by-step protocols for T cell research
Get acquainted with our protocols and antibody panels covering all workflow steps in your T cell research.
MBTP 60: Immunophenotyping of CAR T cell fitness using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- CAR T cells
- T cells
- Antibody panel
- Application protocol
MBTP 56: Immunophenotyping of human CD4+ and CD8+ T cell activation and differentiation using flow cytometry (panel A)
- Keywords
- Flow cytometry and cell analysis
- Immunology
- human
- T cells
- CD4+ T cells
- CD8+ T cells
- Antibody panel
- Application protocol
MBTP 31: Immunophenotyping of human T cells via transcription factor profiling using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- human
- T cells
- Application protocol
- Antibody panel
MBTP 61: Immunophenotyping of human tumor-reactive T cell functionality using flow cytometry
- Keywords
- Antibody panel
- T cells
- human
- Flow cytometry and cell analysis
- Application protocol
- tumor
- Cancer research
- Immunology
MBTP 26: Immunophenotyping of regulatory T cells from human PBMCs using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- human
- regulatory T cells
- T cells
- Application protocol
- Antibody panel
MBTP 54: Immunophenotyping of human CD4+ and CD8+ T cell activation using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- human
- T cells
- CD4+ T cells
- CD8+ T cells
- Antibody panel
- Application protocol
MBTP 58: Immunophenotyping of human CD4+ and CD8+ T cell activation and differentiation using flow cytometry (panel B)
- Keywords
- Flow cytometry and cell analysis
- Immunology
- CD4+ T cells
- CD8+ T cells
- T cells
- Antibody panel
- Application protocol
MBTP 34: Immunophenotyping of CAR T cell persistence and differentiation in blood samples using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- T cells
- CAR T cells
- Antibody panel
- Application protocol
Rapid antigen-specific T cell enrichment (Rapid ARTE)
- Keywords
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Immunology
- human
- PBMCs
- T cells
- CD4+ T cells
- Application protocol
In vitro expansion of human Pan T cells
- Keywords
- Cell separation
- Flow cytometry and cell analysis
- Cell culture
- Immunology
- human
- PBMCs
- T cells
- Application protocol
Multicolor flow cytometric analysis of T cell subsets from mouse spleen
- Keywords
- Sample preparation
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- T cells
- Application protocol
MBTP 44: Immunophenotyping of CAR T cell effector cytokine production using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- T cells
- CAR T cells
- cytokine-producing cells
- Antibody panel
- Application protocol
MBTP 43: Immunophenotyping of CAR T cell transduction efficiency and viability using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- T cells
- CAR T cells
- Antibody panel
- Application protocol
MBTP 4: Immunophenotyping of tumor-specific CD4+ and CD8+ T cells from a human ovarian tumor using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- human
- tumor
- T cells
- lymphocytes
- CD4+ T cells
- CD8+ T cells
- Antibody panel
- Application protocol
MBTP 55: Immunophenotyping of human CD4+ and CD8+ T cell activation and exhaustion using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- CD4+ T cells
- CD8+ T cells
- T cells
- Antibody panel
- Application protocol
MBTP 28: Immunophenotyping of virus-specific T cells from human blood using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- human
- T cells
- blood
- Antibody panel
- Application protocol
In vitro expansion of mouse CD4+ T cells
- Keywords
- Sample preparation
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- T cells
- CD4+ T cells
- Application protocol
Expansion of antigen-specific cytokine-secreting T cells
- Keywords
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Immunology
- T cells
- memory T cells
- cytokine-producing cells
- Application protocol
Human CD4+CD25+ regulatory T cell isolation, in vitro suppression assay and analysis
- Keywords
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Immunology
- human
- PBMCs
- T cells
- regulatory T cells
- Application protocol
Multicolor flow cytometric analysis of regulatory T cells from mouse spleen
- Keywords
- Sample preparation
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- T cells
- regulatory T cells
- Application protocol
TH1, TH2 and TH17 polarization of naive CD4+ mouse T cells
- Keywords
- Sample preparation
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- T cells
- naive T cells
- Th1 cells
- Th2 cells
- Th17 cells
- Application protocol
MBTP 33: Immunophenotyping of activated T cells within antigen-stimulated human PBMCs using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- T cells
- human
- Antibody panel
- Application protocol
Human CD4+CD25+ regulatory T cell isolation, in vitro expansion and analysis
- Keywords
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Immunology
- human
- PBMCs
- T cells
- regulatory T cells
- Application protocol
MBTP 11: Immunophenotyping of T cells from mouse spleen using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- T cells
- Antibody panel
- Application protocol
Engineering of CAR T cells for research use
- Keywords
- Cell separation
- Flow cytometry and cell analysis
- Cell culture
- human
- PBMCs
- T cells
- CAR T cells
- Application protocol
