Miltenyi Biotec-tested panel 15 (MBTP 15)
This application protocol describes the flow cytometric analysis of neutrophils and eosinophils after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|CD11b||REA592||Neutrophils and eosinophils||VioBlue®||450/50 (violet)|
|Viobility™ 405/520 Fixable Dye||-||Viability||-||525/50 (violet)|
|CD193 (CCR3)||REA122||Eosinophils||PE||585/40 (blue)|
|CD3e||REA606||Cell type exclusion||PE-Vio® 615||655-730 (blue)|
|CD19||REA747||Cell type exclusion||PE-Vio 615||655-730 (blue)|
|CD335 (NKp46)||REA815||Cell type exclusion||PE-Vio 615||655-730 (blue)|
|CD45||REA737||Leukocytes||APC-Vio 770||750LP (red)|
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