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The following is a listing of reagents, instruments, and consumables needed for each step of this protocol. These products are for research use only.
|Column||Max. number of labeled cells||Max. number of total cells||Separator|
Coat a 24-well culture dish with 0.01 % Poly-L-lysine overnight at 37 °C. Wash three times with ddH₂O the following day.
Prepare the following cell culture medium: MACS Neuro Medium containing 2 % MACS NeuroBrew®-21, 1 % penicillin/streptomycin and 0.5 mM L-glutamine, 10 ng/mL Human PDGF-AA, and 10 ng/mL Human FGF-2.
Dissociate mouse neonatal brain using the Neural Tissue Dissociation Kit (P) or Neural Tissue Dissociation Kit (T). Follow the protocol of the kit data sheet.
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