Adult astrocyte characterization, isolation, and cultivation

Dr. Melanie Jungblut, co-author of the paper “Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes” published in Glia recently, will give a presentation and discuss with you about: 

1. Generation of astrocyte-specific antibodies
2. Features of astrocyte cell surface antigen-2 (ACSA-2), e.g. its expression pattern at different developmental stages and in different brain regions compared to other neural cell markers
3. Integration of the Anti-ACSA-2 antibody into a complete workflow to study adult rodent brain – from sample preparation and cell isolation to cultivation and immunofluorescence analysis as well as flow cytometry
4. Isolation of neonatal and adult astrocytes using Anti-ACSA-2 MicroBeads for transcriptome analysis by single cell mRNA sequencing 
   
    

Presenters: Melanie Jungblut, PhD, and Hui Demuth-Zhang, PhD, Miltenyi Biotec, Germany 

This webinar took place on August 7, 2018.  

The on-demand webinars and tutorials are available here.