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Monocytes were isolated from PBMCs using CD14 MicroBeads and subsequently cultured for 7 days with IL-4 and GM-CSF to generate immature Mo-DCs and were, finally, cultured for additional 3 days with TNF-α for maturation. Mature Mo-DCs were stained for expression of CD1a, costimulatory molecules (CD80, CD86), CD83 and HLA-DR.
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