Human GM-CSF

Human GM-CSF

Recombinant human GM-CSF induces the differentiation of granulocytes, monocytes, and macrophages. The hematopoietic cytokine is a crucial part of the immune/inflammatory path and serves as both a survival and activation signal for mature myeloid cells. The recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) has been developed for use in cell culture, differentiation studies, and functional assays.

Applications

Human GM-CSF can be used for a variety of applications including:
  • Cultivation of hematopoietic progenitor cells from human bone marrow in semi-solid medium.
  • In vitro generation of Mo-DCs together with Human IL-4.
  • In vitro differentiation of CD34+ cells towards eosinophils.
  • Migration assays for eosinophils.

Alternative names

CSF2

Background information

GM-CSF is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF is a potent chemoattractant for neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of TF-1 cells (NIBSC 88/646)
  • premium grade: ≥ 5×
    10
    6
    IU/mg
    (typical activity: ≥ 1.2×
    10
    7
    IU/mg
    )
  • research grade: ≥ 2×
    10
    6
    IU/mg
  • Selected references

    1. Kaebisch R. et al. (2014) Helicobacter pylori cytotoxin-associated gene A impairs human dendritic cell maturation and function through IL-10-mediated activation of STAT3. J. Immunol. 192: 316-323
    2. Ulfman, L. H. et al. (2008)
      Homeostatic intracellular-free Ca
      2+
      is permissive for Pap1-mediated constitutive activation of α
      4
      integrins on eosinophils.
      J. Immunol. 180: 5512-5519
    3. Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
    4. Guery L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
    5. Koning F. A. et al. (2009) Defining APOBEC3 expression patterns in human tissues and hematopoietic cell subsets. J. Virol. 83: 9474-9485
    6. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
    7. Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
    8. Chase A. J. et al. (2011)
      Impairment of CD4
      +
      T cell polarization by dengue virus-infected dendritic cells.
      J. Infect. Dis. 203: 1763-1774
    9. Brault, J. et al. (2014)
      Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
      ex vivo
      models of X
      0
      -linked, AR22
      0
      - and AR47
      0
      - chronic granulomatous diseases.
      Biores Open Access 3(6): 311-326
    10. Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928
  • Customer reports and application notes

  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 7
E. coli
10 µg
NOK 800,00 
E. coli
50 µg
NOK 1 880,00 
E. coli
10 µg
NOK 1 040,00 
E. coli
50 µg
NOK 2 315,00 
E. coli
100 µg
NOK 4 195,00 
E. coli
500 µg
NOK 12 675,00 
E. coli
1000 µg
NOK 19 470,00 

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