Clone:
REAL926
Type of antibody:
Releasable fluorochromes, Primary antibodies, Recombinant antibodies
Applications:
MICS, IHC, IF
Alternative names:
CEACAM8, CD67, CGM6, NCA-95

Specifications for CD66b Antibody, anti-human, REAdye_lease™

Overview

Clone REAL926 is an antibody fragment derived from the full CD66b antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAdye_lease Complex to bind markers with high avidity.
Clone REAL926 recognizes the human CD66b antigen, a glycosylphosphatidylinositol (GPI) linked protein which is also known as Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8). CD66b is exclusively expressed on neutrophils and eosinophils and on leukocytes of chronic myeloid Leukemia patients and bone marrow. Under normal conditions, neutrophils have minimal expression of CD66b, but incubation in vitro with inflammatory agonists rapidly increases this expression. Furthermore, expression of CD66b in eosinophils is enhanced in vivo in patients with a helminth infection, specifically with Schistosoma mansoni. The eosinophil expression of CD66b is also enhanced in vitro by incubation with activated lymphocytes or by brief incubation with IL-5. Crosslinking of CD66b on peripheral blood neutrophils mediates the release of IL-8 from intracellular storage.
For removal of REAdye_lease fluorochromes for optional relabeling with different fluorochrome-conjugated REAdye_lease antibodies use the REAlease Support Kit (130-120-675).

Alternative names

CEACAM8, CD67, CGM6, NCA-95

Detailed product information

Technical specifications

CloneREAL926
Clonalitymonoclonal
Isotype controlControl Antibody
Hosthuman cell line
Type of antibodyReleasable fluorochromes, Primary antibodies, Recombinant antibodies
Specieshuman
AntigenCD66b
Alternative names of antigenCEACAM8, CD67, CGM6, NCA-95
Distribution of antigengranulocytes, other, eosinophils, neutrophils, leukemia cells, bone marrow

Resources for CD66b Antibody, anti-human, REAdye_lease™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD66b Antibody, anti-human, REAdye_lease™

Publications

  1. Berling, B. et al. (1990) Cloning of a carcinoembryonic antigen gene family member expressed in leukocytes of chronic myeloid leukemia patients and bone marrow. Cancer Res. 50(20): 6534-6539
  2. Eades-Perner, A. M. et al. (1998) Mice transgenic for the human CGM6 gene express its product, the granulocyte marker CD66b, exclusively in granulocytes. Blood 91(2): 663-672
  3. Yoon, J. et al. (2007) CD66b regulates adhesion and activation of human eosinophils. J. Immunol. 179(12): 8454-8462

Related products for
CD66b Antibody, anti-human, REAdye_lease™

1 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?