Clone:
REA144
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
SIRPα, Bit, Mfr, MYD-1, P84, Ptpns1, SHPS-1, Sirp

Extended validation for CD172a (SIRPα) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA144
15-414-
148+
SE5A5+
Cells were incubated with an excess of purified unconjugated CD172a (SIRPα) (REA144) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD172a (SIRPα). Human peripheral blood granulocytes were stained with CD172a (SIRPα) antibodies and with a suitable counterstaining. As a control, CD172a (SIRPα) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD172a (SIRPα). Human peripheral blood granulocytes were stained with CD172a (SIRPα) antibodies and with a suitable counterstaining. As a control, CD172a (SIRPα) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD172a (SIRPα). Human peripheral blood granulocytes were stained with CD172a (SIRPα) antibodies and with a suitable counterstaining. As a control, CD172a (SIRPα) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD172a (SIRPα). Human peripheral blood granulocytes were stained with CD172a (SIRPα) antibodies and with a suitable counterstaining. As a control, CD172a (SIRPα) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD172a (SIRPα). Human peripheral blood granulocytes were stained with CD172a (SIRPα) antibodies and with a suitable counterstaining. As a control, CD172a (SIRPα) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD172a (SIRPα). Human peripheral blood granulocytes were stained with CD172a (SIRPα) antibodies and with a suitable counterstaining. As a control, CD172a (SIRPα) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD172a (SIRPα). Human peripheral blood granulocytes were stained with CD172a (SIRPα) antibodies and with a suitable counterstaining. As a control, CD172a (SIRPα) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD172a (SIRPα) (REA144). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD172a (SIRPα) (REA144). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD172a (SIRPα) (REA144). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD172a (SIRPα) (REA144). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD172a (SIRPα) (REA144). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD172a (SIRPα) Antibody, anti-human, REAfinity™

Overview

Clone REA144 recognizes CD172a, which is also known as signal regulatory protein α (SIRPα). CD172a belongs to the Ig superfamily and is 75–110 kDa single-pass type I regulatory membrane protein. Expression of CD172a is found primarily on hematopoietic stem cells, neurons, and myeloid cells such as macrophages, monocytes, dendritic cells, granulocytes, and myeloid progenitors. CD172a phosphorylated at intracellular tyrosine sites, in response to extracellular stimuli, serves as the docking site for phosphatases such as SHP-1, SHP-2, or SHIP, which inturn desphosphorylate molecules involved in regulating various physiological effects. CD47 has been suggested as a ligand of CD172a.
Additional information: Clone REA144 displays negligible binding to Fc receptors.

Alternative names

SIRPα, Bit, Mfr, MYD-1, P84, Ptpns1, SHPS-1, Sirp

Detailed product information

Technical specifications

CloneREA144
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD172a (SIRPα)
Alternative names of antigenSIRPα, Bit, Mfr, MYD-1, P84, Ptpns1, SHPS-1, Sirp
Molecular mass of antigen [kDa]52
Distribution of antigenmacrophages, dendritic cells, monocytes, neurons, stem cells, granulocytes, hematopoietic stem and progenitor cells
Entrez Gene ID140885
RRIDAB_2904781, AB_2801815, AB_2801813, AB_2811495, AB_2811491, AB_2801909, AB_2655599, AB_2655600, AB_2655603, AB_2655604, AB_2655609, AB_2655610, AB_2904784, AB_2904783, AB_2922085, AB_2922052, AB_2904782

Resources for CD172a (SIRPα) Antibody, anti-human, REAfinity™

Certificates

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References for CD172a (SIRPα) Antibody, anti-human, REAfinity™

Publications

  1. Motegi, S. et al. (2003) Role of the CD47-SHPS-1 system in regulation of cell migration. EMBO J. 22(11): 2634-2644
  2. Jung, C. J. et al. (2016) Comparative analysis of piggyBac, CRISPR/Cas9 and TALEN mediated BAC transgenesis in the zygote for the generation of humanized SIRPA rats. Sci Rep 6: 31455
  3. Oshima, K. et al. (2002) SHPS-1, a multifunctional transmembrane glycoprotein. FEBS Lett. 519(1-3): 1-7

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