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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
We offer synergistic and fully translational solutions for every step of the Research CAR T Cell Workflow.
For the automated T cell transduction process on the CliniMACS Prodigy®, please refer to GMP-engineered T cells.
The first step of CAR T cell production is the enrichment of T cells from your starting material of choice: Use our classical MACS® MicroBeads and Isolation Kits to isolate highly pure T cell subsets (e.g. CD4+, CD8+, or Pan T cells) from human PBMCs or our innovative StraightFrom® Technology to isolate cells directly from whole blood and blood products (e.g. buffy coat, Leukopak®, and LRSC).
With StraightFrom MicroBeads, highly pure T cells are directly enriched in only 30 minutes by skipping time consuming density gradient centrifugation. This novel approach is automatable using the MultiMACS™ Cell24 Separator Plus to increase throughput and productivity.
MACS Technology guarantees gentle and highly efficient T cell isolation, is fully compatible with any downstream application, and allows for a seamless translation into clinics, e.g., on the CliniMACS® Prodigy Platform.
*Leukopak is a registered trademark of StemExpress, LCC.
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Activation of T cells is important to start the proliferation process and to facilitate efficient retroviral or lentiviral transduction with a given CAR construct.
MACS Handbook:
Cell culture
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Transgene expression depends on gene transfer efficiency. However, viral transduction of suspension cells with retroviral vectors is often inefficient and requires the use of transduction enhancers.
MACS Handbook:
Cell culture
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Optimal cultivation and expansion of transduced T cells rely on the strong synergy of T cell activation reagents, TexMACS™ Medium, and MACS® Cytokines.
MACS Handbook:
Cell culture
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To monitor immune cell composition throughout the whole production process and to finally verify functionality of engineered T cells in vitro, we offer a broad spectrum of antibodies and cytokine analysis kits.
T cell characterization
CAR T cell analysis – Functional cytokine profiling
The accurate quantification of cytokines facilitates detailed examination of newly engineered CAR T cells.
MACS Handbook:
Flow cytometry basics
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MACSPlex Cytokine 12 Kit, human
MACSPlex Cytototoxic T/NK Cell Kit, human
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