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We offer synergistic and fully translational solutions for every step of the Research CAR T Cell Workflow.
For the automated T cell transduction process on the CliniMACS Prodigy®, please refer to GMP-engineered T cells.
The first step of CAR T cell production is the enrichment of T cells from your starting material of choice: Use our classical MACS® MicroBeads and Isolation Kits to isolate highly pure T cell subsets (e.g. CD4+, CD8+, or Pan T cells) from human PBMCs or our innovative StraightFrom® Technology to isolate cells directly from whole blood and blood products (e.g. buffy coat, Leukopak®, and LRSC).
With StraightFrom MicroBeads, highly pure T cells are directly enriched in only 30 minutes by skipping time consuming density gradient centrifugation. This novel approach is automatable using the MultiMACS™ Cell24 Separator Plus to increase throughput and productivity.
MACS Technology guarantees gentle and highly efficient T cell isolation, is fully compatible with any downstream application, and allows for a seamless translation into clinics, e.g., on the CliniMACS® Prodigy Platform.
*Leukopak is a registered trademark of StemExpress, LCC.
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Activation of T cells is important to start the proliferation process and to facilitate efficient retroviral or lentiviral transduction with a given CAR construct.
MACS Handbook:
Cell culture
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Transgene expression depends on gene transfer efficiency. However, viral transduction of suspension cells with retroviral vectors is often inefficient and requires the use of transduction enhancers.
MACS Handbook:
Cell culture
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Optimal cultivation and expansion of transduced T cells rely on the strong synergy of T cell activation reagents, TexMACS™ Medium, and MACS® Cytokines.
MACS Handbook:
Cell culture
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To monitor immune cell composition throughout the whole production process and to finally verify functionality of engineered T cells in vitro, we offer a broad spectrum of antibodies and cytokine analysis kits.
T cell characterization
CAR T cell analysis – Functional cytokine profiling
The accurate quantification of cytokines facilitates detailed examination of newly engineered CAR T cells.
MACS Handbook:
Flow cytometry basics
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MACSPlex Cytokine 12 Kit, human
MACSPlex Cytototoxic T/NK Cell Kit, human
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