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Derivation of primary cell cultures from solid tumor material involves multiple critical handling steps. To improve this complex procedure, we developed a workflow allowing for rapid and flexible tissue preparation for subsequent derivation of primary tumor cell cultures using our novel Pancreas TumorMACS™ Medium.
The workflow enables highly standardized cell line derivation and still offers the opportunity to seed dissociated tumors and/or selected cell fractions such as isolated tumor cells or tumor subpopulations.
The gentleMACS™ Dissociator in combination with the Tumor Dissociation Kit, human ensures rapid and standardized dissociation of tissue samples into single-cell suspensions. No matter the tumor entity, gentleMACS Technology provides excellent cell viability and functionality for further downstream cell separation, analysis, and culture.
The Pancreas TumorMACS™ Medium has been developed for derivation and expansion of human tumor cell cultures from pancreatic tumors. The medium is optimized to generate primary cell cultures and cell lines from both primary patient tumors as well as xenotransplanted tumors. Properties of the generated cell cultures closely resemble the parental tumor, including expression of subtype-specific markers, cellular heterogeneity, genetic as well as epigenetic signatures, and tumorigenic potential.