TCRγ/δ Antibody, anti-human

Name: TCRγ/δ Antibody, anti-human
Availability: InStock

TCRγ/δ Antibody, anti-humanExtended Validation

Clone:

11F2

Type of antibody:

Primary antibodies

Isotype:

mouse IgG1κ

Applications:

FC

Alternative names:

TRD, TCRD, TCRDV1, TRD@, TCRG, TRG@, TRG

Flow cytometry applications forTCRγ/δ Antibody, anti-human

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 770
PerCP-Vio 700
VioBlue

Conjugate:

APC

Recommended dilution:

1:50

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-114-026, 130-113-500

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCRγ/δ antibodies as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Extended validation for TCRγ/δ Antibody, anti-human

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with 11F2
B1	+
REA591	++
IMMU510	++
Cells were incubated with an excess of purified unconjugated TCRγ/δ (11F2) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-TCRγ/δ. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-TCR/γδ antibodies and with a suitable counterstaining. As a control, Anti-TCRγ/δ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (11F2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using TCRγ/δ (11F2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TCRγ/δ Antibody, anti-human

Overview

The T cell receptor is a heterodimeric glycoprotein associated with the CD3 antigen. The TCR consists of an α and a β chain (TCRα/β) or a γ and a δ chain (TCRγ/δ). Clone 11F2 reacts with a framework epitope of the γ/δ T cell–receptor. The γ and δ TCR chains are composed of constant and variable regions, each encoded by distinct gene segments. The γ chain forms either disulfide-linked or non-disulfide-linked heterodimers with the δ-subunit.The γ/δ Tcell–receptor is present on a subset of T lymphocytes in peripheral blood, intestinal epithelium, lymph node, thymus, and spleen. TCR γ/δ is involved in the recognition of certain bacterial, self-CD1 molecule, and tumor antigens bound to MHC class I. γ/δ T cells are mainly CD4 negative and CD8 negative. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, innate immune response for some tumor cells, and autoimmune disease. Antigen presentation by γ/δ T cells has been reported.

Alternative names

TRD, TCRD, TCRDV1, TRD@, TCRG, TRG@, TRG

Detailed product information

Technical specifications

Clone	11F2
Clonality	monoclonal
Isotype	mouse IgG1κ
Isotype control	Isotype Control Antibody, mouse IgG1
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	TCRγ/δ
Alternative names of antigen	TRD, TCRD, TCRDV1, TRD@, TCRG, TRG@, TRG
Distribution of antigen	T cells
Entrez Gene ID	6965
RRID	AB_2733699, AB_2733904, AB_2733905, AB_2733462, AB_2733463, AB_2733976, AB_2733977, AB_2733287, AB_2733288, AB_2751186, AB_2751120, AB_2733075, AB_2733076, AB_2733574, AB_2733575, AB_2733698

Resources for TCRγ/δ Antibody, anti-human

Certificates

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References for TCRγ/δ Antibody, anti-human

Publications

Spencer, C. T. et al. (2008) Only a subset of phosphoantigen-responsive gamma9delta2 T cells mediate protective tuberculosis immunity. J Immunol 181(7): 4471-4484
Voogt, P. J. et al. (1989)
Normal hematopoietic progenitor cells and malignant lymphohematopoietic cells show different susceptibility to direct cell-mediated MHC-non-restricted lysis by T cell receptor
^–
/CD3
^–
, T cell receptor gamma delta
⁺
/CD3
⁺
and T cell receptor-alpha beta
⁺
/CD3
⁺
lymphocytes.
J Immunol 142(5): 1774-1780
Borst, J. et al. (1988) Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody. J. Exp. Med. 167(5): 1625-1644
Lanier, L. L. et al. (1987)
The T cell antigen receptor complex expressed on normal peripheral blood CD4
^–
, CD8
^–
T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer.
J. Exp. Med. 165(4): 1076-1094
Conrad, M. L. et al. (2007) TCR and CD3 antibody cross-reactivity in 44 species. Cytometry A 71(11): 925-933
Lanier, L. L. et al. (1988) Structural and serological heterogeneity of γ/δ T cell antigen receptor expression in thymus and peripheral blood. Eur. J. Immunol. 18(12): 1985-1992
Testi, R. and Lanier, L. L. (1989) Functional expression of CD28 on T cell antigen receptor gamma/delta-bearing T lymphocytes. Eur. J. Immunol. 19(1): 185-188
Lanier, L. L. et al. (1987) The gamma T-cell antigen receptor. J. Clin. Immunol. 7(6): 429-440
Moens, E. et al. (2011) IL-23R and TCR signaling drives the generation of neonatal Vγ9 Vδ2 T cells expressing high levels of cytotoxic mediators and producing IFN-γ and IL-17. J. Leukoc. Biol. 89(5): 743-752

Applications

Extended validation