Background information
Brn2 belongs to the POU-III class of neural transcription factors which recognize the octameric DNA-binding sequence ATGCAAAT. Class III POU genes are expressed predominantly in the CNS where they play a role in neurogenesis. In pluripotent stem cell research, overexpression of Brn2 in combination with other transcription factors (e.g. Myt1l, Mash1/Ascl1) has been used to induce neuronal differentiation.
Detailed procedure
For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.
Quality description
StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
