StainExpress™ CAR T Transduction Cocktail, human
StainExpress™ CAR T Transduction Cocktail, human
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CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). | |
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 View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |  View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |
C: | D: |
 View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |  View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |
E: | F: |
 View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |  View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |
G: | H: |
 View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |  View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |
CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). | |
A: | B: |
View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). | View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |
C: | D: |
View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). | View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |
E: | F: |
View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). | View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |
G: | H: |
View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). | View details StainExpress™ CAR T Transduction Cocktail, humanFigure 1CAR T cells, generated within 12 days using the CliniMACS Prodigy ® T Cell Transduction process, were stained with the StainExpress CAR T Transduction Cocktail, CD19 CAR Detection Reagent, human, Biotin, and Biotin Antibody, PE, REAfinity. Staining was carried out at 19−25 °C for 10 minutes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. To exclude debris, a gate was set on FSC versus SSC encompassing all cells (A). To exclude residual erythrocytes and to identify leukocytes, CD45 was used to gate on CD45 + leukocytes (B). Dead cells were excluded by gating on 7-AAD − cells (C). CD3 + cells were identified (D) and discriminated in CAR + and CAR − cells (E). CAR + cells were further divided into CD4 + and CD8 + T cells (F), as were CAR − cells (G). Among CD3 − cells, residual monocytes were defined by CD14 expression (H). |
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