Clone REAL248 is an antibody fragment derived from the full Siglec-H antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL248 recognizes the mouse siglec-H antigen, a 34 kDa transmembrane protein of the Ig superfamily known as mouse sialic acid-binding immunoglobulin-like lectin. Siglec-H is specifically expressed on mouse plasmacytoid dendritic cells, a subset of CD11c
+ dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues. Binding of antibodies to siglec-H inhibits type I interferon production, which can be induced in plasmacytoid dendritic cells by DNA and RNA viruses.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.
