Clone:
delta G9
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2bκ
Applications:
ICFC
Alternative names:
PRF1, FLH2, HPLH2, P1, PFN1, PFP

Extended validation for Perforin Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Perforin. Human peripheral blood mononuclear cells (PBMCs) were first stained with Viobility™ Fixable Dye followed by a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by intracellular staining with Perforin antibodies. As a control, Perforinantibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for Perforin Antibody, anti-human

Overview

The clone delta G9 reacts with human perforin, a 70 kDa cytolytic protein, which is expressed by cytotoxic T lymphocytes and natural killer (NK) cells, and which is stored in cytoplasmic granules. Upon cell activation perforin is released and functions as one of the major effector molecule to mediate killing of target cells.

Alternative names

PRF1, FLH2, HPLH2, P1, PFN1, PFP

Detailed product information

Technical specifications

Clonedelta G9
Clonalitymonoclonal
Isotypemouse IgG2bκ
Isotype controlIsotype Control Antibody, mouse IgG2b
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenPerforin
Alternative names of antigenPRF1, FLH2, HPLH2, P1, PFN1, PFP
Molecular mass of antigen [kDa]59
Entrez Gene ID5551
RRIDAB_2819519, AB_2811588, AB_2811567, AB_2653179, AB_10839415, AB_2653182, AB_2819554

Resources for Perforin Antibody, anti-human

Certificates

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Perforin Antibody, anti-human

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