PepTivator® Dengue Virus Type 4 Protein NS2a

The PepTivator
®
Dengue Virus Type 4 Protein NS2a is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the sequence of the Dengue Virus Type 4 Protein NS2a. The sequence is derived from UniProt Acc. no. P09866 and covers aa 1128–1349 of the genome polyprotein.
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Specifications for
PepTivator
®
Dengue Virus Type 4 Protein NS2a

Overview

The PepTivator
®
Dengue Virus Type 4 Protein NS2a is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the sequence of the Dengue Virus Type 4 Protein NS2a. The sequence is derived from UniProt Acc. no. P09866 and covers aa 1128–1349 of the genome polyprotein.
In vitro
stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Detailed product information

Background information

Dengue virus can be found in tropical and subtropical areas worldwide and belongs to the family of flaviviruses. Dengue fever is a mosquito transmitted viral infection and can be caused by four distinct serotypes (DEN-1, DEN-2, DEN-3, and DEN-4), inducing high fever, severe muscle pain, and nausea. Recovery from infection by one Dengue virus induces immunity against the homologous serotype but not against the other three serotypes. Secondary infection with a different DENV serotype can cause the potentially life-threatening dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS).

Downstream applications

PepTivator
®
Dengue Virus Type 4 Protein NS2a – research grade has been specifically developed for efficient
in vitro
stimulation of Dengue–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4
+
and CD8
+
T cells in various applications, including:
  • Detection and analysis of antigen-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
  • Isolation of viable antigen-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
  • Generation of antigen-specific effector/memory T cells from naive T cell populations.
  • Pulsing of antigen-presenting cells.

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