PepTivator
^®
CMV pp65

Name: PepTivator® CMV pp65
Availability: InStock

PepTivator
^®
CMV pp65

PepTivator

^®

CMV pp65 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the pp65 protein of cytomegalovirus (UniProt ID: P06725).

PepTivator peptide pools have been developed for the efficient

in vitro

stimulation of antigen-specific CD4

⁺

and CD8

⁺

T cells, as peptides of 15–aa length with 11–aa overlap represent an optimized solution for stimulating both CD4

⁺

and CD8

⁺

T cells in various applications.

Quantitative, phenotypical, or functional analysis of pp65-specific T cell immunity can provide important information on the natural course of

Product data and images for PepTivator
^®
CMV pp65

Figures

Figure 1

Production of IFN-γ by human CD4
⁺
or CD8
⁺
T cells after stimulation with different antigens using PepTivator Peptide Pools. Data from two different donors are shown (triplicates).

Figure 1

Production of IFN-γ by human CD4

⁺

or CD8

⁺

T cells after stimulation with different antigens using PepTivator Peptide Pools. Data from two different donors are shown (triplicates).

Specifications for PepTivator
^®
CMV pp65

Overview

PepTivator

^®

CMV pp65 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the pp65 protein of cytomegalovirus (UniProt ID: P06725).

PepTivator peptide pools have been developed for the efficient

in vitro

stimulation of antigen-specific CD4

⁺

and CD8

⁺

T cells, as peptides of 15–aa length with 11–aa overlap represent an optimized solution for stimulating both CD4

⁺

and CD8

⁺

T cells in various applications.

Quantitative, phenotypical, or functional analysis of pp65-specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.

PepTivator CMV pp65-HT is a peptide pool lyophilized in the wells of a 96-well plate, allowing stimulation and analysis of cells directly on the plate.

The convenient 96-well format, composed of 12 individually removable strips of 8 wells each allows for easy and flexible experimental set-up, different antigens can be combined by assembling different strips. A control can also be added easily by using strips from the Control plate (12x8).

Detailed product information

Background information

CMV pp65 (65 kDa lower matrix phosphoprotein), also known as glycoprotein 64 or UL83, is a virion tegument protein and the main component of the enveloped subviral particle. CMV pp65 is an immunodominant target of CD4

⁺

and CD8

⁺

T cell responses to CMV. CMV pp65-specific T cells predominantly produce inflammatory cytokines, such as IFN-γ, IL-2, and TNF-α.

Downstream applications

The

in vitro

stimulation of pp65-specific CD4

⁺

and CD8

⁺

T cells with PepTivator CMV pp65 causes the secretion of effector cytokines and the upregulation of activation markers, which then allow the detection and isolation of pp65-specific T cells

^1-6

:

Detection and analysis of CMV pp65-specific CD4⁺ and CD8⁺ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular staining, or other technologies.
Isolation of viable CMV pp65-specific CD4⁺ T cells with the CD154 MicroBead Kit, or of CD4⁺ and CD8⁺ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay - Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
Generation of CMV pp65-specific CD4⁺ and CD8⁺ effector/memory T cells from naive T cell populations.
Pulsing of antigen-presenting cells for research on dendritic cell vaccination.

Resources for PepTivator
^®
CMV pp65

Documents and Protocols

App notes/customer reports

Efficient and rapid in vitro generation of fully functional multi-virus-specific CD4⁺ and CD8⁺ T cells.

App notes/customer reports

Detection, enrichment, and direct phenotypic and functional characterization of antigen-specific naive and memory T cell subsets.

References

PepTivator Peptide Pools - selected references

References for PepTivator
^®
CMV pp65

Publications

Peggs, K. S. et al. (2011) Directly selected cytomegalovirus-reactive donor T cells confer rapid and safe systemic reconstitution of virus-specific immunity following stem cell transplantation. Clin. Infect. Dis. 52(1): 49-57
Beck, B. et al. (2011) Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission. J. Transl. Med. 9: 151
Aïssi-Rothé, L. et al. (2010) Rapid generation of full clinical-grade human antiadenovirus cytotoxic T cells for adoptive immunotherapy. J Immunother 33(4): 414-424
Faist, B. et al. (2010) Cytomegalovirus infection- and age-dependent changes in human CD8+ T-cell cytokine expression patterns. Clin. Vaccine Immunol. 17(6): 986-992
Zandvliet, M. et al. (2010) Co-ordinated isolation of CD8(+) and CD4(+) T cells recognizing a broad repertoire of cytomegalovirus pp65 and IE1 epitopes for highly specific adoptive immunotherapy. Cytotherapy 12: 933-944
Dodero, A. et al. (2009) Haploidentical stem cell transplantation after a reduced-intensity conditioning regimen for the treatment of advanced hematologic malignancies: posttransplantation CD8-depleted donor lymphocyte infusions contribute to improve T-cell recovery. Blood 113: 4771-4779

Data and images

Data and images for PepTivator
^®
CMV pp65

Figures

Figure 1

Production of IFN-γ by human CD4

⁺

or CD8

⁺

T cells after stimulation with different antigens using PepTivator Peptide Pools. Data from two different donors are shown (triplicates).

Specifications

Specifications for PepTivator
^®
CMV pp65

Overview

PepTivator

^®

CMV pp65 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the pp65 protein of cytomegalovirus (UniProt ID: P06725).

PepTivator peptide pools have been developed for the efficient

in vitro

stimulation of antigen-specific CD4

⁺

and CD8

⁺

T cells, as peptides of 15–aa length with 11–aa overlap represent an optimized solution for stimulating both CD4

⁺

and CD8

⁺

T cells in various applications.

Quantitative, phenotypical, or functional analysis of pp65-specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.

PepTivator CMV pp65-HT is a peptide pool lyophilized in the wells of a 96-well plate, allowing stimulation and analysis of cells directly on the plate.

The convenient 96-well format, composed of 12 individually removable strips of 8 wells each allows for easy and flexible experimental set-up, different antigens can be combined by assembling different strips. A control can also be added easily by using strips from the Control plate (12x8).

Detailed product information

Background information

CMV pp65 (65 kDa lower matrix phosphoprotein), also known as glycoprotein 64 or UL83, is a virion tegument protein and the main component of the enveloped subviral particle. CMV pp65 is an immunodominant target of CD4

⁺

and CD8

⁺

T cell responses to CMV. CMV pp65-specific T cells predominantly produce inflammatory cytokines, such as IFN-γ, IL-2, and TNF-α.

Downstream applications

The

in vitro

stimulation of pp65-specific CD4

⁺

and CD8

⁺

T cells with PepTivator CMV pp65 causes the secretion of effector cytokines and the upregulation of activation markers, which then allow the detection and isolation of pp65-specific T cells

^1-6

:

Detection and analysis of CMV pp65-specific CD4⁺ and CD8⁺ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular staining, or other technologies.
Isolation of viable CMV pp65-specific CD4⁺ T cells with the CD154 MicroBead Kit, or of CD4⁺ and CD8⁺ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay - Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
Generation of CMV pp65-specific CD4⁺ and CD8⁺ effector/memory T cells from naive T cell populations.
Pulsing of antigen-presenting cells for research on dendritic cell vaccination.

Resources

Resources for PepTivator
^®
CMV pp65

Documents and Protocols

App notes/customer reports

Efficient and rapid in vitro generation of fully functional multi-virus-specific CD4⁺ and CD8⁺ T cells.

App notes/customer reports

Detection, enrichment, and direct phenotypic and functional characterization of antigen-specific naive and memory T cell subsets.

References

PepTivator Peptide Pools - selected references

References

References for PepTivator
^®
CMV pp65

Publications

Peggs, K. S. et al. (2011) Directly selected cytomegalovirus-reactive donor T cells confer rapid and safe systemic reconstitution of virus-specific immunity following stem cell transplantation. Clin. Infect. Dis. 52(1): 49-57
Beck, B. et al. (2011) Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission. J. Transl. Med. 9: 151
Aïssi-Rothé, L. et al. (2010) Rapid generation of full clinical-grade human antiadenovirus cytotoxic T cells for adoptive immunotherapy. J Immunother 33(4): 414-424
Faist, B. et al. (2010) Cytomegalovirus infection- and age-dependent changes in human CD8+ T-cell cytokine expression patterns. Clin. Vaccine Immunol. 17(6): 986-992
Zandvliet, M. et al. (2010) Co-ordinated isolation of CD8(+) and CD4(+) T cells recognizing a broad repertoire of cytomegalovirus pp65 and IE1 epitopes for highly specific adoptive immunotherapy. Cytotherapy 12: 933-944
Dodero, A. et al. (2009) Haploidentical stem cell transplantation after a reduced-intensity conditioning regimen for the treatment of advanced hematologic malignancies: posttransplantation CD8-depleted donor lymphocyte infusions contribute to improve T-cell recovery. Blood 113: 4771-4779

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^®
CMV pp65

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PepTivator® CMV pp65

PepTivator® CMV pp65

Product data and images for PepTivator® CMV pp65

Figures

Figure 1