Mouse LIF

LIF stands for leukemia inhibitory factor. Mouse LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Mouse LIF

Figures

Figure 1

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Mouse LIF activity assay.
The biological activity of Mouse LIF was determined by inhibition assay using mouse M1 cells. Activity of Mouse LIF, premium grade (red line) was compared to another commercially available product (black line) with fully equivalent results.

Figure 1

Mouse LIF activity assay.
The biological activity of Mouse LIF was determined by inhibition assay using mouse M1 cells. Activity of Mouse LIF, premium grade (red line) was compared to another commercially available product (black line) with fully equivalent results.

Figure 2

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SDS-PAGE of Mouse LIF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Mouse LIF, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

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Mass spectrometry analysis (ESI-MS) of Mouse LIF, premium grade. The peak corresponds to the calculated molecular mass of 19862 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Mouse LIF, premium grade. The peak corresponds to the calculated molecular mass of 19862 Da.

Specifications for Mouse LIF

Overview

LIF stands for leukemia inhibitory factor. Mouse LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse LIF can be used for a variety of applications including:
  • Maintenance of self-renewal and pluripotency in conventional mouse ESC cultures.

Detailed product information

Background information

LIF is a pleiotropic cytokine, which is critically involved in embryonic development and blastocyst implantation. LIF belongs to the interleukin 6 family and functions through the gp130 activation of STAT3. In mice LIF is a key factor that prevents embryonic stem cells (ESC) to differentiate. Additionally, LIF affects hematopoiesis, neural development, bone and energy metabolism, and inflammation.

Biological activity

  • Inhibition of M1 cells
  • research grade: ≥ 1×
    10
    6
    U/mg
  • premium grade: ≥ 2×
    10
    6
    U/mg
    (Typical specific activity: ≥ 2.5×
    10
    6
    U/mg
    )
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Mouse LIF

Documents and Protocols

Brochures/posters

MACS® Cytokines - Mouse LIF for reliable ESC culture

Background information

Premium-grade cytokine benefits

App notes/customer reports

Use of magnetically enriched pluripotent stem cells increases chimerism rate after blastocyst injection and enables the use of inbred ES cell lines for tetraploid complementation.

App notes/customer reports

How to switch to optimal activity-based cytokine dosing

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Mouse LIF

Publications

  1. Rose, T. M. and Bruce, A. G. (1991) Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Proc. Natl. Acad. Sci. U.S.A. 88: 8641-8645
  2. Junyent, S. et al. (2021) Wnt- and glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division. Elife 10: e59791
  3. Heurtier, V. et al. (2019) The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Nat Commun. 10(1): 1109
  4. Lee, Y. X. F. et al. (2019) Considerations and Implications in the Purification of Extracellular Vesicles - A Cautionary Tale. Front Neurosci 13: 1067
  5. Owens, N. et al. (2019) CTCF confers local nucleosome resiliency after DNA replication and during mitosis. Elife 8: e47898
  6. Zhang, S. et al. (2019) Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells. Nat Commun. 10(1): 496
  7. Lowndes, M. et al. (2016) Immobilized WNT Proteins Act as a Stem Cell Niche for Tissue Engineering. Stem Cell Reports 7(1): 126-137
  8. Walter, M. et al. (2016) An epigenetic switch ensures transposon repression upon dynamic loss of DNA methylation in embryonic stem cells. Elife 5: e11418
  9. Barral, S. et al. (2013)
    Efficient neuronal
    in vitro
    and
    in vivo
    differentiation after immunomagnetic purification of mESC derived neuronal precursors.
    Stem Cell Res. 10(2): 133-46

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