Mouse IL-3 IS

Name: Mouse IL-3 IS
Availability: InStock

Mouse IL-3 IS

IL-3 IS stands for interleukin 3 "Improved Sequence". Mouse IL-3 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Product data and images for Mouse IL-3 IS

Figures

Figure 1

**Mouse IL-3 IS activity assay.**
The biological activity of Mouse IL-3, premium grade, was determined by proliferation assay using 32D cells.

Figure 1

Mouse IL-3 IS activity assay.

The biological activity of Mouse IL-3, premium grade, was determined by proliferation assay using 32D cells.

Figure 2

**SDS-PAGE of Mouse IL-3 IS, research grade**
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Mouse IL-3 IS, research grade

under reduced (R) and non-reduced (NR) conditions.

Figure 3

Mass spectrometry analysis (ESI-MS) of Mouse IL-3 IS, premium grade. The peak corresponds to the calculated molecular mass of 15068 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Mouse IL-3 IS, premium grade. The peak corresponds to the calculated molecular mass of 15068 Da.

Specifications for Mouse IL-3 IS

Overview

IL-3 IS stands for interleukin 3 "Improved Sequence". Mouse IL-3 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-3 IS can be used for a variety of applications, including:

As cell culture supplement to maintain hematopoietic cells or cell lines, such as the pro-B lymphoid cell line BaF/3 or 32Dcl3 cells.
Induction of colony formation of hematopoietic progenitor cells in semi-solid medium, e.g. of highly purified Sca-1⁺/lin^- cells.
Pre-stimulation of hematopoietic progenitor cells for retroviral transduction prior to bone marrow transplantation.
In vitro differentiation studies, e.g. of mast cells.

Detailed product information

Background information

Interleukin 3 (IL-3) is a hematopoietic growth factor which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biologic activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

Biological activity

Proliferation of 32D cells (NIBSC 91/662)
research grade: ≥ 2×
10
⁷
U/mg
premium grade: ≥ 3×
10
⁷
U/mg
We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Mouse IL-3 IS

Documents and Protocols

Background information

Premium-grade cytokine benefits

App notes/customer reports

How to switch to optimal activity-based cytokine dosing

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for Mouse IL-3 IS

Publications

Rodriguez, S. et al. (2011) The SKP2 E3 ligase regulates basal homeostasis and stress-induced regeneration of HSCs. Blood 117(24): 6509-6519
Rochelle, T. et al. (2013) p210bcr-abl induces amoeboid motility by recruiting ADF/destrin through RhoA/ROCK1. FASEB J. 27(1): 123-134

Data and images