Purest mitochondria preparation. Mitochondria were prepared by different protocols. Amounts of COX I (mitochondria) and KDEL (endoplasmic reticulum) in the mitochondrial fractions obtained using differential centrifugation (DC), Mitochondria Isolation Kit, human, and ultracentrifugation (UC) were analyzed by Western blotting.
Compared to DC and UC, mitochondria isolated with the Mitochondria Isolation Kit, human have no detectable contamination with ER. (Courtesy of Dr. H.-T. Hornig-Do, Newcastle, UK)