The TriM Scope™ II is a modular platform for intravital multiphoton microscopy and optogenetic manipulation, which can be equipped with a variety of lasers, scanner configurations, detectors, and dedicated modules for in vivo imaging. Thanks to its modularity, the system can be adapted to various applications, including deep tissue multicolor imaging, intravital 3D time-lapse measurements, and calcium monitoring under natural and biased conditions. Thus, the TriM Scope II Multiphoton Microscope can be used for a large variety of applications, ranging from developmental biology to immuno-oncology and neuroscience.
Scanner options for the TriM Scope II Multiphoton Microscope
Resonant scanner | Galvo scanner | ||
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Unidirectional scan | Bidirectional scan | ||
Scan frequency | 8 kHz | 1.2 kHz | 2.0 kHz |
Frame rate | 30 fps at 512×512 pixels | 2.3 fps at 512×512 pixels | 3.3 fps at 512×512 pixels |
Scan field (with 20× objective) | 500 µm × 500 µm | 500 µm × 500 µm |
Detector options for the TriM Scope II Multiphoton Microscope
GaAs "red" | GaAsP | Multialkali "blue" | Multialkali "green/red" | |
---|---|---|---|---|
Quantum efficiency at peak wavelength | 12% at 800 nm | 40% at 580 nm | 25% at 375 nm | 18% at 480 nm |
Spectral response | 380–890 nm | 300–720 nm | 230–870 nm | 230–920 nm |
Sensitivity adjustment range | 1:50 | 1:50 | 1:10,000 | 1:10,000 |
Camera options for the TriM Scope II Multiphoton Microscope
sCMOS camera | CMOS camera | |
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Sensor type | Front-illuminated scientific CMOS | Front-illuminated CMOS |
Sensor format | 4.2 megapixels | 4.2 megapixels |
Active pixels (w × h) | 2048×2048 | 2048×2048 |
Pixel size | 6.5 µm × 6.5 µm | 5.5 µm × 5.5 µm |
Sensor size | 13.3 mm × 13.3 mm, 18.8 mm diagonal | 11.26 mm x 11.26 mm , 15.9 mm diagonal |
Pixel well depth | 45,000 e- | 13,500 e- |
Pixel frame rate | 40 fps (100 fps optional) | 80 fps |
Readout mode | Rolling shutter | Global shutter |
Read noise [median] rolling shutter | 2.1 e- | 13e- |
Interface | USB 3.1 (Camera Link optional) | USB 3.0 |
Quantum efficiency | 80% | >50% |
Laser options for the TriM Scope II Multiphoton Microscope
Wavelength (nm) | Models (examples) | |
---|---|---|
Ultra-wide tunable | 680–960, 960–1300, 1025 | CRONUS laser |
680–1300 | Spectra Physics InSight® X3+, Coherent® Chameleon Discovery NX TPC | |
Ti:sapphire | 690–1080 | Coherent® Chameleon Ultra II, Spectra Physics MaiTai® |
OPO | 1010–1340 | Coherent® Chameleon MPX (pumped with Ultra II) |
OP(CP)A | 1300–1700 | Class 5 Photonics White Dwarf, Light Conversion® I-OPA, Spectra Physics® Spirit-NOPA®-VISIR |
Fixed-wavelength femtosecond | 920, 1040, 1064 | Coherent® Axon Series, Spark Lasers® Alcor Series |
Visible | 405, 445, 488, 514, 532, 552 (others on request) | Coherent® OBIS™ |
Explore the different modules below and discover how to customize the instrument for your research.
The TriM Scope II Multiphoton Microscope can be equipped with detection modules for multiple detectors in epifluorescence, as well as in transmission mode. In epifluorescence mode, the detection port can be equipped with adapters for two, three, four, or six PMTs, enabling a flexible combination of GaAsP, GaAs, and/or Bialkaline PMTs. The six-fold PMT adapter is the most advanced setup, and enables the best spectral discrimination (Spectral Detector). The Transmission Detector module incorporates up to two PMTs. Software integration for each module ensures an easy-to-use approach with maximum utilization of the PMT options.
Specifications for the Treatment Scanner
Scan frequency | Up to 2.5 kHz |
Scan speed (with 20× Objective) | Up to 1.25 µm/µs (line treatment) |
Scan field (with 20× Objective) | 500 µm × 500 µm |
Wavelength range | 400–1300 nm |
Laser coupling | Fiber (recommended) or free-air |
Specifications for the Cloud Scanner
Number of foci | 4 |
Length of line patter (with 20× Objective Lens) | ~2 µm |
Time delay between foci | ~100 ps |
Wavelength range | 720-1050 nm (up to 1300 nm upon request) |
Specifications for Adaptive Optics
Aperture (diameter) | 15 mm |
Channels | 37 |
Working frequency | Up to 1 kHz |
Specifications for In Vivo Stabilization
Compensation speed | Up to 250 frames/s |
Accuracy | <50 nm |
Response time | <2 ms |
Travel range | Ø 40 μm (lateral), 48 μm (axial) |
Software | Complete integration into ImSpector with readout of actual and corrected position |
Specifications for the Endomicroscope
Magnification | 1.5–1.9× (depending on image working distance) |
Numerical aperture | 0.28 |
Working distance | 200 µm |
Field of view (diameter) | Ø 100 µm |
Tip diameter | 0.42 mm (PI) or 0.5 mm (stainless steel) |
Tip length | 10 mm (including mantle) 3 mm (at 0.42 mm or 0.5 mm diameter) |
Excitation spectral range | 800–1300 nm |
Detection spectral range | 420–800 nm |
Side view angle | 90° |
Specifications for Intravital Stage
Dimension plate | 537 mm × 450 mm (w × d) |
Height z | 190–270 mm |
Maximum travel range | x: 50 mm, y: 50 mm, z: 80 (180) mm |
Resolution xy | 0.078 µm |
Repetition accuracy | 1.0 µm |
Maximum speed | up to 4.4 mm/s |
Top plate | magnetic stainless steel, with mounts for sample holder and a 25 mm × 25 mm thread (M6) array |
Specifications for Horizontal Imaging
Sample rotation | 0–360 ° |
Detection options | Up to 6 PMTs (in forward- and/or epi-direction) |
Scan field (with 20× objective) | 500 µm × 500 µm |
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