The MACS Freezing Solution is a ready-to use serum-free and animal component-free media formulation developed for the cryopreservation of primary cells and solid tissues. The MACS Freezing Solution ensures high preservation of viability and recovery of cells and tissues after thawing.

Data and images for
MACS
®
Freezing Solution

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Figure 1

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Analysis of cryopreserved PBMCs. Peripheral blood mononuclear cells (PBMCs) from 3 different donors were cryopreserved for 2 weeks in liquid nitrogen, either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution.
After this time period, cells were thaw and cell recovery (A) and cell viability (B) were analyzed by flow cytometry using propidium iodide staining and a MACSQuant Analyzer 10. Cell recovery is calculated as the percentage of viable cells recovered after thawing with reference to the amount of fresh cells that were cryopreserved.

Figure 1

Analysis of cryopreserved PBMCs. Peripheral blood mononuclear cells (PBMCs) from 3 different donors were cryopreserved for 2 weeks in liquid nitrogen, either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution.
After this time period, cells were thaw and cell recovery (A) and cell viability (B) were analyzed by flow cytometry using propidium iodide staining and a MACSQuant Analyzer 10. Cell recovery is calculated as the percentage of viable cells recovered after thawing with reference to the amount of fresh cells that were cryopreserved.

Figure 2

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Analysis of cryopreserved tumor cell suspensions. CT26 mouse tumors were dissociated using the Tumor Dissociation Kit, mouse in combination with the gentleMACS Octo Dissociator with Heaters. The resulting tumor cell suspensions were cryopreserved for 2 weeks in liquid nitrogen either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution.
After this time period, cells were thawed and cell recovery (A) and cell viability (B) were analyzed by flow cytometry using propidium iodide staining and a MACSQuant Analyzer 10. Cell recovery is calculated as the percentage of viable cells recovered after thawing referred to the amount of fresh cells that were cryopreserved.

Figure 2

Analysis of cryopreserved tumor cell suspensions. CT26 mouse tumors were dissociated using the Tumor Dissociation Kit, mouse in combination with the gentleMACS Octo Dissociator with Heaters. The resulting tumor cell suspensions were cryopreserved for 2 weeks in liquid nitrogen either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution.
After this time period, cells were thawed and cell recovery (A) and cell viability (B) were analyzed by flow cytometry using propidium iodide staining and a MACSQuant Analyzer 10. Cell recovery is calculated as the percentage of viable cells recovered after thawing referred to the amount of fresh cells that were cryopreserved.

Figure 3

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Analysis of cryopreserved tumor samples. CT26 mouse tumors were cut into small pieces and cryopreserved for 2 weeks in liquid nitrogen either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution. After this time period, tumor pieces were thaw and immediately dissociated using the Tumor Dissociation Kit, mouse in combination with the gentleMACS Octo Dissociator with Heaters.
The resulting cell suspensions were analyzed to determine the cell count (A) and cell viability (B) by flow cytometry using propidium iodide staining and a MACSQuant Analyzer 10. For the latter, the cell count obtained from the dissociation of fresh tumors is shown as a reference.

Figure 3

Analysis of cryopreserved tumor samples. CT26 mouse tumors were cut into small pieces and cryopreserved for 2 weeks in liquid nitrogen either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution. After this time period, tumor pieces were thaw and immediately dissociated using the Tumor Dissociation Kit, mouse in combination with the gentleMACS Octo Dissociator with Heaters.
The resulting cell suspensions were analyzed to determine the cell count (A) and cell viability (B) by flow cytometry using propidium iodide staining and a MACSQuant Analyzer 10. For the latter, the cell count obtained from the dissociation of fresh tumors is shown as a reference.

Figure 4

View details
Analysis of the cellular composition of cryopreserved PBMCs. Peripheral blood mononuclear cells (PBMCs) from 3 different donors were cryopreserved for 2 weeks in liquid nitrogen, either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution.
After this time period, cells were thawed and the cellular composition was analyzed by flow cytometry using the 8-color Immunophenotyping Kit, human and a MACSQuant Analyzer 10. Analysis of the fresh PBMC samples is shown as a reference.

Figure 4

Analysis of the cellular composition of cryopreserved PBMCs. Peripheral blood mononuclear cells (PBMCs) from 3 different donors were cryopreserved for 2 weeks in liquid nitrogen, either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution.
After this time period, cells were thawed and the cellular composition was analyzed by flow cytometry using the 8-color Immunophenotyping Kit, human and a MACSQuant Analyzer 10. Analysis of the fresh PBMC samples is shown as a reference.

Figure 5

View details
Analysis of the cellular composition of cryopreserved tumor cell suspensions. CT26 mouse tumors were dissociated using the Tumor Dissociation Kit, mouse in combination with the gentleMACS Octo Dissociator with Heaters. The resulting tumor cell suspensions were cryopreserved for 2 weeks in liquid nitrogen either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution.
After this time period, cells were thawed and the cellular composition was analyzed by flow cytometry using REAfinity antibodies and a MACSQuant Analyzer 10. Analysis of the fresh tumor cell suspensions is shown as a reference.

Figure 5

Analysis of the cellular composition of cryopreserved tumor cell suspensions. CT26 mouse tumors were dissociated using the Tumor Dissociation Kit, mouse in combination with the gentleMACS Octo Dissociator with Heaters. The resulting tumor cell suspensions were cryopreserved for 2 weeks in liquid nitrogen either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution.
After this time period, cells were thawed and the cellular composition was analyzed by flow cytometry using REAfinity antibodies and a MACSQuant Analyzer 10. Analysis of the fresh tumor cell suspensions is shown as a reference.

Figure 6

View details
Analysis of the cellular composition of cryopreserved tumor samples. CT26 mouse tumors were cut into small pieces and cryopreserved for 2 weeks in liquid nitrogen either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution. After this time period, tumor pieces were thawed and immediately dissociated using the Tumor Dissociation Kit, mouse in combination with the gentleMACS Octo Dissociator with Heaters.
The resulting cell suspensions were analyzed to determine the cellular composition by flow cytometry using REAfinity antibodies and a MACSQuant Analyzer 10. Analysis of the freshly dissociated tumor samples is shown as a reference.

Figure 6

Analysis of the cellular composition of cryopreserved tumor samples. CT26 mouse tumors were cut into small pieces and cryopreserved for 2 weeks in liquid nitrogen either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution. After this time period, tumor pieces were thawed and immediately dissociated using the Tumor Dissociation Kit, mouse in combination with the gentleMACS Octo Dissociator with Heaters.
The resulting cell suspensions were analyzed to determine the cellular composition by flow cytometry using REAfinity antibodies and a MACSQuant Analyzer 10. Analysis of the freshly dissociated tumor samples is shown as a reference.

Specifications for
MACS
®
Freezing Solution

Overview

The MACS Freezing Solution is a ready-to use serum-free and animal component-free media formulation developed for the cryopreservation of primary cells and solid tissues. The MACS Freezing Solution ensures high preservation of viability and recovery of cells and tissues after thawing.

Detailed product information

Background information

Cryopreservation of living cells and tissues is frequently applied for long term storage to preserve the samples for later use. The freezing and thawing of the samples are critical steps in the cryopreservation process which can influence the recovery of viable cells after storage. The MACS Freezing Solution allows the cryopreservation of primary cells and solid tissue samples under serum-free conditions with high preservation of viability and recovery of cells and tissues after thawing. It has been tested and validated on diverse cell types and tissues, such as human and mouse tumor samples, peripheral blood mononuclear cells (PBMCs), and cells from dissociated solid tissues (e.g. cells from dissociated tumors). MACS Freezing Solution contains 10% DMSO as cryoprotectant.

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Freezing Solution

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