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Aliquot 1 of cultured MSCs was stained with the MSC Phenotyping Cocktail and aliquot 2 with the Isotype Control Cocktail. The fractions were analyzed by flow cytometry using the MACSQuant®
Analyzer 10. Each histogram was overlaid with the corresponding isotype control to identify positively stained cells. In order to distinguish between activated MSC and non-MSCs, the HLA-DR antibody (conjugated to different fluorochromes) has been used in combination with other MSC-negative markers.
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